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III. Genetics: 0 <br />A. Propagation program developed around sound genetic concepts. <br />(Genetic Management Guidelines For Listed and Candidate Fish Species in <br />Region 6) will provide the conceptual framework for genetic management. <br />B. Genetic risk assessment. <br />C. Genetic baseline sampling and tracking. <br />IV. Broodstocks: <br />A. Wild brood fish. <br />B. Captive broodstock. <br />C. Founder stock. <br />D. Identification of individual brood fish. <br />E. Breeding strategy. <br />F. Holding facilities. <br />G. Nutrition. <br />V. Spawning and Incubation: <br />A. Use of hormones including type, dosage, timing, etc. <br />B. Anesthetics. <br />C. Spawning techniques. <br />D. Egg and melt handling procedures during fertilization, water hardening, egg 41 <br />enumeration, chemical treatment, etc. <br />F. Incubation techniques including type of unit, water flow rates, temperature, <br />treatments, length of incubation time. <br />G. Data collection and record keeping. <br />VI. Rearing: <br />A. Type of rearing units. <br />B. Water type, flow rates, temperature, quality, gas levels, etc. <br />C. Fish density. <br />D. Nutrition. <br />E. Inventory, and other records. <br />F. Fish health-relate to Fish Health Plan. <br />VII. Marking and Tagging: <br />A. All marks and tags will be described in the Monitoring Plan. <br />B. Anesthetics. <br />C. Equipment. <br />D. Personnel needs. <br />E. Evaluation of tag retention. 0