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other planning documents. • <br />SPAWNING. INCUBATION AND REARING <br />Identify and characterize the culture parameters needed to allow the species to reproduce and <br />grow, including spawning and rearing methods used and rearing conditions, equipment, and <br />techniques utilized to fulfill the hatchery culture program for early life stages and <br />broodstock. Injection procedures and schedules, hormones, and anesthetics need to be <br />documented. Additionally, egg and milt processing, egg enumeration, chemical treatments, <br />temperature data, and incubation procedures should be described. Pertinent_.information such <br />as female size, volume of eggs, egg size, total egg number, eggs per female;per cent hatch, <br />fry size, stocking densities, and survival should be noted. Any other incubation and rearing <br />characteristics need to be documented, as necessary. <br />SURROGATE SPECIES <br />Many listed and candidate species have related species that are very similar in many respects, <br />such as taxonomy, morphology, physiology, behavior, food habits, and habitat. They may <br />exhibit very similar genetic profiles, and frequently hybridize. Hybridization could present a <br />threat to the survival of related species through genetic swamping if the hybrids are fertile, <br />and through competition for a limited habitat. These close relationships have lead many <br />experts to conclude that the more abundant species can be used as a surrogate species when <br />conducting nutritional, rearing, spawning, handling and other cultural trials; thus, eliminating <br />the need to jeopardize the less abundant species. Methods tested and proven on the surrogate <br />species must then be phased in on the listed or candidate species. <br />INTRODUCTION AND MONITORING <br />An introduction section will be prepared when management determines that captive <br />propagation and introduction is necessary for the restoration or recovery of the species. The <br />Service will initiate the planning process for the management areas, and plan development <br />will be a cooperative partnership process between the Service, States, other federal agencies, <br />tribes, and private interest groups. The introduction section needs to be reviewed by the <br />restoration and recovery team and approved by the Regional Director. The team must <br />designate and prioritize specific stocking sites, and make recommendations on numbers, <br />timing, and sizes of fish to be stocked at any one of the specific sites (See Introduction <br />Guidelines). <br />All fish will be tagged or marked for later identification. Stocked areas will be assessed <br />(monitored) by management offices to determine survival, growth, movement, and habitat <br />selection, plus other attributes of the ecosystem such as relative abundance of other fish <br />species encountered and biological status of the target populations. This requires that the <br />population be described, indicating the general health or condition of the population, whether •