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2. Inventory data which may be appropriate for developing <br />relationships, develop testable hypotheses, and analyze data. <br />3. Select relationships which suggest potential limiting <br />conditions(i.e., significant changes in dependent variable as a <br />function of independent variable; Fig. 1). <br />4. Identify data needs (fill gaps with professional judgment until <br />empirical relationships can be developed). <br />a. List data needs for important variables and life history phases. <br />b. Propose hypotheses relating life stage response to variables. <br />c. Test hypotheses, develop relationships. <br />III. Develop, verify, and validate relationships and methods for quantifying <br />species response to flow alterations. Focus on limiting variables and <br />limiting life stages; document assumptions. <br />A. Life stage response vs. flow (Fig. 2A). <br />6. Life stage response vs. variable (Fig. 26). <br />C. Variable vs. flow (Fig. 2C). <br />7