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32. Annually maintain and operate two facilities as fiuanobsck chub <br />refuaia and to produce a minimum of 20,000 ht,++T~back chubs <br />through 1990). 10,000 roundtail chubs, 10,000 boaytail chubs, <br />and their hybrid crosses (through 1985). <br />When two propagation facilities are achieved (one sew facility <br />and the Willow Beach National Fish Hatchery) it will be possible <br />to produce adequate nutabers of humpback and other chubs. 'Most <br />facilities will incur their major costs during construction or <br />modification; day-to-day operation costa will be relatively <br />constant throughout the expected •1Z year life span. The Fiah <br />and Wildlife Service, Bureau of Reclamation, and certain state <br />wildlife agencies will share the responsibility for funding the <br />operation of propagation facilities. These two facilities will <br />also serve as refugia for the humpback chub populations. <br />33. Develop propagation and holding techniQues to maximize production <br />of yo ~ and maintain adult brood stocks. <br />Most of the necessary technology will be developed in situ under <br />controlled conditiana. Specific problems are treating diseased <br />brood fish successfully, inducing maturation of the gonads, and <br />simply keeping brood fish alive and healthy over a Period of <br />years. <br />34. While maintaining genetic integrity. produce_or acquire <br />and maintain the following brood stocks• 10 pairs of humpback <br />chubs in each of the two facilitiea• 10 airs of roumdtaii chubs <br />in each facility. 5 pairs of bonytail chubs at the new facility, <br />and 10 pairs of bonytail chubs at Willow Beach. <br />The necessary. size of the brood stocks in each hatchery will <br />depend upon their fecundity and the culturist's success in <br />fish- i.e., adequate technology and manpower. <br />producing yo~g s <br />The above numbers of fish are estimates. Adults and subadults <br />(replacement brood fish) will comprise the total brood stock. <br />Equal sex ratios may be used. <br />21 <br />