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critical to some phase of their life cycle. In order to meet these <br />objectives and limit bias, a systematic sampling program was developed <br />with a randomized start. This ensured the equal pursuit of these fishes <br />across the full spectrum of habitats throughout the study area and <br />permitted interpretation of results independent of bias. Sampling <br />program design and detailed methods of acquiring data are detailed in <br />Appendix A. <br />Other field investigations addressed specific facets of the target <br />fish's life histories and environmental needs. These were directed at <br />describing spawning areas, migratory behavior, prevalence of disease, <br />tolerances to natural and artificial chemical compounds, interspecific <br />competition, speciation, and striped bass predation. <br />Many difficulties were encountered in studying the fish fauna of <br />this large river system because of large areas encompassed, large flows <br />encountered and inaccessability of certain reaches. Water turbitity <br />further complicated the study by precluding any possibility of directly <br />observing various aspects of these fishes behavior or location, therefore, <br />all hypotheses ventured had to be extrapolations from fish captures <br />using a variety of devices; some active, others passive. One solution <br />that was adopted to help resolve this problem was to directly observe <br />northern squawfish which inhabits streams of much greater clarity in the <br />Columbia River Basin. The Idaho Cooperative Fishery Unit was enlisted <br />to conduct those in situ behavioral studies of the northern squawfish as <br />a surrogate to the Colorado squawfish. <br />Program objectives also included areas of investigation that could <br />be dealt with only in a laboratory setting. These included such areas <br />as swimming stamina, temperature tolerance and preferences, total <br />dissolved solids tolerance and preferences, and bioassay tests for <br />potentially toxic trace elements. These investigations were contracted <br />to the Utah and Idaho Cooperative Fishery Units at Logan, Utah and <br />Moscow, Idaho. <br />A third area of investigation included in the study program was the <br />culture of these species both to fulfill the need for laboratory specimens <br />and to advance cultural technologies for them. These activities were <br />centered at the Willow Beach National Fish Hatchery with satelite programs <br />at the Utah State Fisheries Experiment Station and Dexter National Fish <br />Hatchery. These programs dealt with the use of hormones to induce <br />spawning, incubation of eggs and rearing of fry and older fish. <br />A fourth area of study that received some attention was the issue <br />of taxonomic classification of the three endemic Gila species. The <br />appropriate taxonomic designation of these fish has been clouded by much <br />variability between specimens and hybridi2ation. A review of the <br />meristic characteristics of these fish by our field staff and seveFal <br />noted taxonomists was undertaken along with a cytogenetic karyotyp`~'of ~- <br />the various Gila spp. by a scientist at Utah State University. <br /> <br />