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~ ~ <br />' piling of eggs made this technique less than optimum. A tctal of 1,315 fry <br />were obtained from this female. Additional injections yielded only nonviable <br />eggs. <br />Female ~3 and 4 - These two females received 300 and 500 IU respectively <br />and lathough each yielded a few eggs every day or two there were no viable <br />eggs. <br />Injections of 2 to 8 mg carp pituitary injected every other day into <br />four females gave no results. <br />Four females that were seined from the pond. One was injected with 2 mg <br />carp pituitary/lb. daily, and after four injections the female died before <br />any-eggs were ovulated. One female was used in the circular tank spawning <br />test. The two remaining females were injected with 300 IU and 500 IU inter- <br />muscularly daily for two days and the third day injected with the same dosage <br />rate but in the body cavity. These two females were ripe and running eggs on <br />the morning of the fourth day. Eggs were not enumerated to reduce handling. <br />Estimated numbers were 60,000 eggs each. Eggs were placed in trays in a <br />Heath incubator. A total of 28,500 swim-up fry was obtained from these two <br />females. All eggs water hardened but two and three day old egg samples showed <br />that 32X were developing. Fungus in the incubator reduced the number of fry <br />obtained. <br />Egg Measurements <br />Eggs were enumberated using Von Bayer's method. Egg counts were made <br />from both water hardened eggs and non-water hardened eggs. The eggs from <br />the females during the first part of the spawning tests were of various <br />stages. In some instances over 50y of the eggs would 'absorb no water. The <br />data presented here will be from lots of eggs that all had water hardened. <br />The data from non-water hardened eggs will not be presented due to the <br />11 <br />