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.;~ ~ .: <br />' with 300 to 500 IU with injections every third to fifth day. *lales that were <br />dry at the first injections were brought to fluidity with injections of 300 IUs <br />daily for three or four days. The response of the females that was held in <br />tanks for a period of 14 days before injections wereinitiatedgave a very poor <br />response. The females would give a few eggs daily but all were nonviable. <br />It appeared that the reabsorbtion process had started. The female that re- <br />ceived 800 IU for three every other day injections had strippable eggs 24 <br />hours after the third injection. The eggs that were stripped and fertilized <br />from this female gave strong evidence that this dosage was too strong. Over <br />half of the eggs wer immature and would not even water harden. About 10% of <br />the eggs appeared to be viable after water hardening. The remainder were <br />eggs that appeared to be overripe, This female yielded 139,000 eggs. The <br />eggs were one of•the lots that were placed in jars for hatcing. Premature <br />hatching made fry enumeration and hatching success impossible. <br />Female 4~1 that injections started with five days after arrival and in- <br />jected every other day with 500 IU yielded 9,000 eggs the day following second <br />injection. All eggs water hardened. These eggs were not coated with clay but <br />was placed in a :ScDonald jar and allowed to stick to the jar and were incubated. <br />A total of 1,800 fry were hatched from this lot of eggs. A~ eggs that lost <br />their adherence were prematurely hatched. The eggs appeared to loose much of <br />their adherence at about twoof a e. Injections continued with this <br />female and from 1,000 to 5,000 eggs could be stripped but all eggs were non- <br />viable. <br />Female ~~2 was injected with 300 IU every other day and the day after the <br />third injection had ovulated eggs that were stripped and water hardened. This <br />female yielded a total of 32,300 eggs. These eggs all water hardened correctly <br />but less than 10% (9.9) started embryo development. These eggs were incubated <br />on screens in tanks with a water flow over the eggs. Fungus problems and <br />10 <br />