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Last modified
7/14/2009 5:02:32 PM
Creation date
6/1/2009 12:00:57 PM
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UCREFRP
UCREFRP Catalog Number
7970
Author
Dowling, T. E. and W. L. Minckley.
Title
Genetic Diversity Of Razorback Sucker As Determined By Restriction Endonuclease Analysis Of Mitochondrial DNA.
USFW Year
1994.
USFW - Doc Type
Bureau of Reclamation, # 0-FC-40-09530-004,
Copyright Material
NO
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from randomly generated data indicates significant haplotype frequency differences among <br />populations. <br />8, an analog of FST, was calculated using the program HAPLOID (Weir, 1990), <br />providing a measure of the proportion of variation distributed among populations. Values <br />range from 0 to 1, with higher values indicative of greater isolation. The hypothesis 8~ (i.e., <br />no population subdivision) was tested by jackknifing across samples. In jackknifing, one <br />population is removed and A calculated from the remaining samples. Each population is <br />removed in turn until 8 is calculated for all possible combinations. The mean and standard <br />deviation of this set of values provides the statistical test; populations exhibit significant <br />difference in allele frequenciPS if the confidence interval for 8 does not include 0. <br />Gene diversity analysis (Nei, 1987) was performed using the program REAP (McElroy <br />et al., 1992), partitioning the total average estimate of sequence divergence among all <br />individuals (total nucleotide diversity) into within- and among-population components <br />(population nucleotide diversity and nucleotide divergence, respectively). When <br />subpopulations aze connected by considerable gene exchange, most of the variation is found <br />within populations (i.e., diversity is large and divergence small). When subpopulations are <br />isolated, however, moss of the variation is partitioned among populations (i.e., diversity is <br />small and divergence large). <br />The extent of gene flow among populations was also inferred from haplotype networks <br />(Slatkin and Maddison, 1989) generated in two ways: 1) estimates of sequence divergence <br />were obtained among all possible pairwise combinations of restriction site haplotypes (Nei, <br />1987), and the resulting distance matrix was used to cluster haplotypes using the UPGMA <br />option in NTSYS (Sokol, 1992); and 2) haplotypes were grouped using variable restriction <br />sites (presence/absence) in a parsimony analysis providee~ by PAUP (Swofford, 190). <br />6 <br />
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