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Definitive Toxicity Test No. III <br />. Chemical tested: Copper (CuSO4) J.T. Baker Chemical Co.; lot no. 703144. <br />' Stock .solution: 1,000 mg/1. Prepared by dissolving cupric sulfate, <br />anhydrous, mol. wt. 159.b0. Solubility 14.3 grams in 0 C water per <br />100 ml. Calculations: CuSO4 159.60 X <br />a <br />. Cu 63.546 liter <br />R ~ 2.5116 g CuSO4/liter = 1000 mg/1 Cu <br />in solution. <br />Test protocol: <br />Conc. in mg/1 No. fish/conc. Vol. stock solution <br />- 0.5 20 0.25 ml <br />0.7 20 0.35 <br />0.9 20 0.45 <br />1.1 20 O.SS <br />1.3 20 0.65 <br />Fish: <br />Fish were postlarval northern squawfish that had absorbed their yolk <br />sac. These fish were collected on 7/9/80 as eggs, adhering to rocks, in <br />a pool of the St. Joe River 1-2 miles downstream from the Calder Bridge. <br />This pool contained a spawning aggregation of mature squawfish. Many of <br />the eggs hatched almost immediately when handled. Hatched and unhatched <br />eggs were placed in a plastic bucket and aerated at 17 C in a blend of U of I <br />and St. Joe River water. On 7/18/80, 70x of the water was removed and <br />replaced with TSWB. The fish, were transferred on 7/30/80 by 5 ml pipets <br />with suction bulbs.to 1.9-liter canning jars containing 500 ml TSWB at 17 C. <br />~ The fish were never fed. Testing was begun 8/1/80 at 0830. Details concerning <br />fish load, dissolved oxygen and pH levels are given in Appendix Table 1. <br />Observations were made at 3, 6, I2, 24, 36, 48, 60, 72, 84 and 96 hours <br />~u~ only the 12, 24, 48, 72 and 96 hour data are given in these results. The <br />reaul~s ~~~ g~rein ~?~~ ~ppendi~c Table 2. <br />