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level was dropped. The bottom strand was weighted down with short sections of <br />pipe to keep the device near the pond bottom. The A.V.R.D. was pulled through <br />the water until the amount of aquatic vegetation removed was determined to be <br />negligible enough to effect fish seining. Note that not all aquatic <br />vegetation will be able to be removed from the pond. <br />Fish Seining <br />A variety of large and small seines should be selected to match pond <br />dimensions and depth as water level is dropped. It was found that bag seines <br />were more efficient in fish capture than other types of seines. As the water <br />level drops the seine tends to collapse in vertical height and the bottom of <br />the seine rolls up due to remaining aquatic vegetation. This will undoubtedly <br />allow for some fish escaping. Switching to a smaller seine as needed will <br />alleviate the problem. A careful check for fish rolled up in the aquatic <br />vegetation captured should be conducted when cleaning the seine. This is <br />especially true when working with fish smaller than 100mm. In general, large <br />seines were moved slowly through the pond to keep the net on the bottom. When <br />the water level was shallow it was found that a fast seine would yield more <br />fish than a slow seine. Once the water level reaches the bottom of the stand <br />pipe, about a foot of water will remain. A portable pump was borrowed from <br />the Ouray Refuge personnel to help siphon out the remaining water. The pump <br />can also speed up the process of dropping the pond level if used in <br />conjunction with the opening of the stand pipe drain. Make sure that the <br />drafting bell is in good shape, or fish will be sucked into the pump. Note <br />that not all of the water will be removed, about 3 to 5 inches will remain. <br />After it has been determined that all fish have been removed from the pond, <br />let the remaining water sit for a day or two undisturbed, then seine again. <br />You will undoubtedly seine out more fish. Follow this procedure until you are <br />absolutely sure that no fish remain. <br />A total of 653 fish were removed from pond #1 between September 1st and <br />3rd, 1988, and ranged in length from 100mm to 271mm. Of these 653 fish, 36 <br />were sent to W.L. Minckley at Arizona State University for morphological, DNA, <br />and electrophoresis studies. The remaining 617 fish were eventually stocked <br />in the Green River. <br />A total of 1322 fish were removed from pond #3 between September 16th and <br />20th, 1988. Of these 1322 fish, 28 were placed in an aquarium at the CRFP <br />office in Vernal, Utah. These fish were never weighed or measured. An <br />additional 2 fish were sent to W.L. Minckley at Arizona State University for <br />morphological, DNA, and electrophoresis studies prior to fin-clipping. <br />Another 21 fish were also lost prior to fin-clipping; 13 fish were sucked into <br />the portable pump during draining, 2 of which were preserved, and another 8 <br />were lost to mortality in the indoor tanks (6 preserved). Also 7 fish were <br />lost in transit to the stocking sites, 6 of which were preserved. Thus 1262 <br />fish ranging in size from 40mm to 120mm were eventually stocked into the Green <br />River. <br />Tank Holding <br />After being seined out of the ponds, the fish were put into 500 gallon <br />circular tanks indoors. The tanks were filled with heated water at a rate of <br />approximately 9 gallons per minute (gpm), and maintained with a constant <br />inflow of 3.5 to 5.5 gpm. An oxygen bottle with air stone was set up to <br />maintain favorable dissolved oxygen levels. Daily records of water