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Laboratory Studies <br />Two experiments will be conducted under laboratory conditions to <br />evaluate largemouth bass feeding behavior. The first experiment will <br />involve testing the preference of largemouth bass for selected forage <br />species , and the second will involve testing the preference of bass <br />for a certain size of squawfish. <br />Squawfish will be obtained from Willow Beach NFH, and bass from <br />either the study ponds, other wild sources, or from the San Marcos <br />National Fish Hatchery and Development Center, Texas. For both exper- <br />iments, three 1.33 m diameter circular stock-watering tanks will be <br />used. These will be held at the Utah Water Research Laboratory in <br />Logan. Each will be equipped with a flow-through system using-dechlor- <br />inated city water heated to 22 degrees C and kept at a depth of 37 cm. <br />Each tank will be enclosed and a 15 watt bulb used to light the inter- <br />ior. Light will be set on a timer to maintain a daylength of 14 hours. <br />When food items are placed in a tank, the bass will be partitioned <br />from them until the preyfish have acclimated to the tank environment <br />(0.25-0.5 h). The partition will then be lifted and the enclosed tank <br />left undisturbed until checked the following day.. <br />To determine whether a bass shows a preference for a particular <br />size of young squawfish, or for a particular species when offered in <br />combination, data will be analysed using analysis of variance with the <br />experiment set up as randomized block design (split-plot) where squaw- <br />fish size or prey'species.is the subplot treatment (Neter and Wasser- <br />man 1974). <br />The design-of the species preference experiment is similar to that <br />used by Espinosa and Deacon (1973) and Lewis et al. (1961). Individual <br />• bass are confined in tanks and offered an equal number of _Aifferent <br />kinds of food items. Those items most readily taken are assumed to be <br />preferred by the bass. One bass of each of three different sizes <br />(160-210 mm TL, 250-275 mm TL, and 330-370 mm TL) will be placed in a <br />separate tank and combinations of two species of forage fish offered <br />in numbers of 5-10 per species. Forage species used will be those most <br />commonly found in Colorado River backwaters or those most predominately <br />found in bass stomachs during the first field season. Colorado squaw- <br />fish of similar size will be one of the two species offered in combin- <br />34