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<br />point of maximum water depth, and at the midpoint toward either side. Total area and total <br />volume of the habitat were estimated from these measurements. Primary and secondary substrate <br />types were recorded at each depth point. Sub-surface temperatures (°C) and turbidity were ~ <br />recorded at the midpoint of each transect. Turbidity was gauged on a subjective scale from 0 (> <br />6" visibility) to 1 (2" to 6" of visibility) and 2 (< 2" of visibility). The turbidity and temperature <br />of the adjacent main channel were taken at the same time. <br />Fish were collected from each habitat using a 1.2 m by 4 m seine, with 1.6 mm mesh size. <br />The number of seine hauls varied from one to three with the size of the habitat. In general, ~ <br />habitats wider than 4 m but less than 25 meters long were seined once, those between 25 and 50 <br />m seined twice, and those > 50 m seined three times. In large habitats, the seine hauls were <br />usually made at the width transects. Habitats less than 4 m wide and 25 m long were completely <br />seined in one pass. Length, width and depth of each seine haul was measured, to estimate effort <br />in area and volume seined. ~ <br />Native fish were identified, measured and released in the field. Colorado pikeminnow that <br />died during collection were preserved in ethanol during the first two years of the study, and were <br />transferred to the Larval Fish Laboratory at Colorado State University (CSU) for future analysis <br />of scales and otoliths. Non-native fishes were identified, separated into adult/subadult categories <br />and counted. For each habitat, catch-per-effort (CPE) in #fish/100 m2 and #fish/100 m3• was ~ <br />estimated by dividing the number of YOY pikeminnow collected by the area and volume <br />sampled in all seine hauls. Mean CPE was estimated for each trip by dividing all pikeminnow <br />collected by the total area and volume sampled. <br />Additional information on water temperature was acquired using Ryan tempmentor <br />temperature loggers set to record mid-column temperatures in the main channel and in selected ~ <br />backwaters where pikeminnow had been collected. Temperatures were taken every two hours. <br />Data from these recorders is not continuous due to equipment loss and failures. <br />Habitat types were classified according to a scheme, developed in 1992 with hydrologist <br />Dr. Jack Schmidt (USU) for a similar study on the Green River (Trammell and Chart 1998), that <br />related types of habitats to the hydrological process that formed them (Table 1). Furthermore, ~ <br />the development of each habitat was related to fluctuating flow levels and can be divided into <br />three stages: areas with some flow (i.e. small side channels and shoreline areas), areas with no <br />flowing water and one end open to the main channel (backwaters), and areas with no access to <br />the main channel (isolated pools). <br />Each sampling trip was coordinated with avideo-imaging overflight conducted by the <br />Bureau of Reclamation (BOR). Individual habitats were mapped onto aerial video prints and <br />overlays to evaluate their persistence through time. Habitat persistence was evaluated beginning <br />with post-runoff habitats and ending in spring prior to the runoff. Habitats that were ephemeral <br />within the year (sampled only once) were rated 0, those which were seasonally stable (sampled <br />twice in one year) rated 1, and those annually stable (from summer to spring) rated 2. ~ <br />Descriptive statistics were used to compare species composition and relative abundance <br />between seasons and the two sections. Pearson's correlations were used to test for significant <br />linear relationships between most variables, including peak runoff flows, sampling flows, habitat <br />area and volume, catch rates ofnon-native cyprinids and Colorado pikeminnow, degree <br />accumulations, total length of Colorado pikeminnow in the fall, and overwinter survival. ~ <br />Multinomial analysis was used to test for habitat selection of Colorado pikeminnow and non- <br />native cyprinids for geomorphically classified habitat types. <br />2 <br />