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Last modified
7/14/2009 5:02:31 PM
Creation date
5/22/2009 7:29:13 PM
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UCREFRP
UCREFRP Catalog Number
7760
Author
Beyers, D. W., R. T. Muth and M. S. Farmer.
Title
Experimental Evidence of Competition Between Larvae of Colorado Squawfish and Fathead Minnow.
USFW Year
1994.
USFW - Doc Type
\
Copyright Material
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experimental unit was an aquarium. Forty-five 76-L aquaria (30 x 75 x 32 cm <br />high) were housed within the same room. Cool-white fluorescent lamps were the <br />only source of illumination, and a 12:12-h light:dark photoperiod was <br />maintained. <br />Water was supplied by a well on the Colorado State University campus and <br />had the following characteristics: dissolved ,oxygen, > 6.1 mg/L; pN, 8.2; <br />temperature, 20 ± 1 °C; alkalinity, 237 mg/L as CaC03; hardness, 344 mg/L as <br />CaC03; and specific conductance, 720 µS/cm. The bottom of each aquarium was <br />covered with approximately 2 cm of washed sand. Each aquarium was aerated <br />continuously with a single airstone and water was added periodically to <br />maintain a depth of 24 cm. <br />Zooplankton abundance levels were based on results of field studies and <br />encompassed a realistic range of zooplankton densities (S. J. Grabowski and <br />S. D. Hiebert, unpublished report). Zooplankton were collected from Fossil <br />Creek Reservoir (Larimer County, Colorado) with an 80-µm-mesh plankton net. <br />Zooplankton were transferred to the laboratory where they were quantified by <br />subsampling and a stock was prepared by diluting to a target density. <br />Aliquots of stock were delivered to aquaria twice daily to obtain 2000 <br />zooplankton per aquarium per feeding (26 zooplankton / L). Feeding-regime <br />levels (32, 80, and 200 zooplankton•fish~~•day~~) were obtained by manipulating <br />number of fish per aquarium rather than number of zooplankton. For example, <br />the 32 zooplankton•fish-~•day~~ treatment was obtained by delivering 2000 <br />zooplankton twice daily into an aquarium containing 125 fish. Numbers of fish <br />per aquarium corresponding to 80 and 200 zooplankton•fish-1•day~~ were 50 and <br />20, respectively. This procedure allowed manipulation of zooplankton relative <br />8 <br />
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