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Colorado, bioconcentration factors for zooplankton range from 267 to 5700 (B.C. Osmundson, <br />pers. comm.). Our relatively simple food chain exposure system did not include many <br />components of natural systems (e.g., sediment). However, the system was not intended to mimic <br />all aspects of natural systems. It was intended to use a natural process to incorporate selenium <br />into the diet of razorback sucker. The magnitude of bioaccumulation in diet of razorback sucker <br />was less than anticipated. To account for this apparent difference in environmental cycling, we <br />have modified exposure conditions in ongoing studies (see below) to prevent depletion of <br />dissolved selenium in algae and rotifer cultures. However, the apparent sensitivity of <br />bioaccumulation rates to cycling of selenium suggests that results of this and other investigations <br />should be interpreted based on concentrations in tissues of organisms and not based on water <br />concentrations. <br />Exposure to contaminants can reduce an organisms ability to obtain food, as well as <br />increase its metabolic demand for energy (Beyers et al. 1999). Lemly (]993b) showed that <br />selenium exposure increases metabolic stress in fish. The growth of fish in this investigation was <br />relatively slow (from 10.6 to 11.5 mm TL in 28 days) suggesting that the quantity of food offered <br />was smaller than the maximum ration that the fish could have consumed. Slow growth can be <br />considered a weakness of this study, but it also suggests that fish had limited resources to offset <br />effects of selenium exposure. Because energy for compensating effects of stress induced by <br />selenium exposure was limited, this investigation may provide a conservative (worst-case) <br />estimate of effects over the concentration ranges studied. Results of this study confirm that <br />exposure to dietary selenium concentrations below 1.4 µg/g dry weight do not adversely effect <br />survival and growth of larval razorback sucker. <br />22 <br />