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Dexter NFH is located in the Pecos River Valley of southeastern <br />New Mexico. Elevation is 3,500 ft, average annual rainfall about <br />12 inches, and the growing season 180-200 days. Water is supplied <br />by three shallow wells, is slightly alkaline (pH 7.2 to 7.5), and <br />temperature is a constant 64° F. Total hardness averages 2,100 <br />ppm and total dissolved solids approximately 3,500 ppm. Culture <br />facilities at Dexter consist of a holding house (with sixteen 350- <br />gal tanks, a hatching battery, and laboratory) and 48 earthen <br />ponds. Ponds vary from 0.1 to 1.8 acres, comprising a total of <br />20.8 acres. Wastewater is impounded on the station and thus is <br />isolated from all other surface waters in the area (Jensen in <br />Rinne et al., in press). <br />PROGRAM OBJECTIVES <br />The program at Dexter NFH was implemented to assist in recovering <br />imperiled southwestern fishes, most of them federally listed. The <br />following objectives were selected to plan and carry out the <br />program: (1) maintain a viable, protected gene pool of imperiled <br />desert fishes, (2) develop techniques for culturing them, (3) <br />study their biological requirements, (4) provide live and <br />preserved fish to cooperating agencies and institutions, (5) <br />provide an exchange of expertise and data on their culture and <br />management, (6) publish findings, and (7) implement a public <br />information program concerning the plight of, and recovery efforts <br />for, imperiled fishes of the region (Jensen 1983). <br />MAINTENANCE OF GENETIC DIVERSITY <br />An important aspect of holding, culturing and reintroducing <br />imperiled fishes is maintenance of genetic variation. The problem <br />regarding the management of any species that is artificially mass- <br />produced or held in artificial (unnatural) habitats is_the <br />perpetuation of the "natural" gene pool (Buth and Murphy 1.984). <br />Does maintenance and production of fish species in artificial <br />habitats and/or artificial propagation result in a loss of <br />variability that would change their genetic makeup or impact their <br />ability to reestablish-self-sustaining "natural" populations once <br />reintroduced into historic habitats? <br />This has been and remains a major concern of the Dexter program <br />and has been answered in part by separate electrophoretic studies <br />on Dexter held and produced fishes. Tony Echelle (pers. comm.), <br />Oklahoma State University, compared simultaneously collected wild <br />and Dexter held Pecos gambusia, and Comanche Springs and Leon <br />-33- <br />