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. . . construction and operation and maintenance costs. The design effort was focused on the 300,000 fish production scenario, with extrapolation of design and cost information to the 150,000 and 600,000 production scenarios. Standardization of the design of the production units was done as much as possible to allow flexibility in production quotas for each of the four species. This flexibility would also permit the facility to be used for propagation of fish, other than the four endangered fish, should the need arise. The initial production quotas were used as a basic criteria for total biomass2 and a target for design which can be related to maximum, average and minimum water flows and various configurations of rearing systems. The general overall size and generic layout of the facility were designed to meet these initial production targets. A state-of-the-art recovery facility was developed from the conceptual modeling effort, incorporating the latest technologies while considering the special needs for propagating rare and endangered species. The design effort included development of a hatchery simulation model which utilized input variables such as water flow rates, water temperature, production goals and site conditions and determined the required number and type of rearing ponds, tanks, feed rates and the general size of the facility. To protect broodstock and refugia stocks from potential disease outbreaks, the TAC recommended that only one-half of all brood and refugia stocks for the four species be held at this facility. The remaining half would held at various other state and federal facilities. Additionally, as established by the T AC, rearing methods for each species were to be equally divided between intensive and extensive culture methods. A layout of the facility configured for a warm well water source and 300,000 fish production level is illustrated on Sheet 1. Facility layouts for the other design combinations are included in the Final Report. A nutritional strategy was developed for extensive culture of augmentation stocks and refugia which is designed to stimulate a natural feeding response as a means of enhancing survival and ultimately genetic diversity. Accommodating this strategy requires natural feed production facilities, which have been incorporated in the design. Nutritional requirements for intensive culture of augmentation stocks will be satisfied through a diet of manufactured feed. Flow requirements for extensive culture are based on oxygen consumption data and an acceptable level of water turnover rate for extensive culture. Flow requirements for intensive culture (using various water types and production scenarios) have been calculated by computer modeling based on oxygen consumption data for Colorado squawfish. Tables 4 and 5 identify the maximum requirements for flow, start tanks and 2 Defined in GI06SIIry. 9