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. . . REFERENCE NUMBER: CITATION: 021 Bulkley, R. V., c.R. Berry, R. Pimental, and T. Black. 1981. Tolerance and preferences of Colorado River endangered fishes to selected habitat parameters. Pages 185-241 In W.H. Miller, JJ. Valentine, and D.L. Archer. (cds.) Colorado River Fishery Project Final Repon: Pan 3, Contract ReportS. U.S. Fish and Wildlife Service, Bureau of Reclamation., Salt Lake City, Utah. SPECIES AND LIFE STAGE: TOPICS: KEYWORDS: SUMMARY: HB, HB_JUV, BT, BT_LAR, CS, CS_LAR, CS_ADULT, RZ. RZ_JUV Water Quality, Transpon, Temperature HUMPBACK CHUB, BONYTAIL, COLORADO SQUAWFISH, RAZORBACK SUCKER, TEMPERATURE PREFERENDUM, EGG TEMPERATURE TOLERANCE, TRANSPORT STRESS Four endangered Colorado River fishes were studied to: determine temperature and total dissolved solid preferences; determine baseline hematological parameters; determine swimming stamina at various water velocities and temperatures; determine effectS of swimming and transpon stress on certain blood parameters. FInal temperature preferendum are as follows: humpback chub 240C, bonytail chub 24.20C, razorback sucker 24.9"C, Colorado squawfish (juvenile) 24.6DC and Colorado squawfish (adult) 25.40C. Egg temperature tolerance was tested for Colorado squawfish, bonytail and humpback chub. Results (temperature DC / percent hatched/ incubation time) are as follows: Colorado squawfish 5DIO%/_ . 10010%/- , 140,1)%/- , 200/60%/10 days Bonytail chub 5DIO%/_. 10010%/- . 140/60%/10 days, 2oonO%/6 days, 260/80%(3 days, 31DIO%/_, 3~1O%/- Humpback chub 5DIO%/_ , 100(30%/19 days, 140/50%/16 days, 2001100%/4 days, 26DI95%!3 days Blood glucose levels in 40 razorback suckers increased from 54 mg/100 ml to 144 mg/100 ml after 1~ hours of transport. No significant change in blood chloride was noted. Salt was suggested as a way of reducing osmoregulatory stress during transport. A-21