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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />METHODS <br /> <br />Field Collections <br /> <br />Sampling for Colorado pikeminnow larvae was done using drift-net sampling at <br />three locations in 1992, at four locations in 1993 and at six locations in 1994, 1995 and <br />1996. The three stations sampled in all five years were: the Colorado River near Loma at <br />RK 247 (RM 153.7); the Colorado River just upstream of the Gunnison confluence at RK <br />275 (river mile 171.2); and the Gunnison River (below Redlands diversion) just upstream <br />of the confluence of the Colorado River (RK 3; RM 1.8). A station located on the <br />Gunnison River above the Redlands Diversion was established in 1993 at RK 8.8 (RM <br />5.5). Third stations were added to both the Colorado and Gunnison rivers in 1994. On <br />the Colorado River it was located upstream of Red lands Parkway bridge at RK 270 (RM <br />167.8) and on the Gunnison River the site was at Bridgeport at RK 47 (RM 29.3). <br /> <br />Sampling was done simultaneously at each site, and was conducted daily at dawn <br />throughout the month of July and into August depending on flow conditions. Passive, <br />near-shore drift-net sampling was conducted using two ichthyoplankton nets deployed <br />perpendicular to the shoreline at depths from 30 to 40 cm, and where velocities ranged <br />from 15 to 45 cm/s. The top of the net was positioned just below the water surface. <br />These nets were 0.5 m diameter, conical plankton nets mounted on 0.5 x 0.3 m <br />rectangular steel frames, and fitted with 33 cm-Iong, removable PVC buckets with a <br />threaded cod end (10 cm diameter). Each net had a 560 micron Nytex nylon mesh a <br />length of 4.0 m and an open-mesh to mouth ratio of 11 : 1. Each net was attached to two <br />steel rods driven into the substrate. Fish samples at all stations except Loma were field <br />processed and preserved in formalin. From 1993 to 1996 fish samples at Loma were <br />preserved in alcohol. Taxonomic identification and enumeration was done by CPMU <br />Larval Fish Lab in Fort Collins. Length was measured to the nearest 0.1 rom on all <br />Colorado pikeminnow larvae and length subsequently was used to estimate the age (days) <br />and the spawning date of each pikeminnow. <br /> <br />Data Analysis <br /> <br />Total drift was estimated for a one hour sample period by dividing the sample <br />count by the estimated proportion of flow sampled while the net was set. The percentage <br />of the total flow sampled was estimated as the ratio of the flow rate through the net to the <br />mean daily flow recorded at the gauge representing the sample site. Water volumes <br />passing through each net were calculated from start and finish velocity readings made with <br />either a Marsh-McBimey (Model 201) current meter or a General Oceanics Model 2030 <br />flow meter suspended within the net mouth. Water temperature was measured at the start <br />of each sampling period. <br /> <br />Individual total length of all Colorado pikeminnow sampled were used in age- <br />growth equations developed by Haynes et al. (1985) to calculate the age (days post- <br />hatching) of each fish at capture. The date of spawning for that larvae was determined by <br /> <br />7 <br />