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<br /> <br />14 <br /> <br />and rapids, with extensive reaches inaccessible except by air or by <br />floating down the river considerable distances. <br /> <br />Since this study area encompasses some 570 miles of the Colorado <br />and Green Rivers, it was impossible to study the entire length with <br />the allocated manpower; therefore, it was deemed necessary to rely on <br />a statistically sound unbiased sampling scheme from which to infer <br />habitat/fishery life history relations. <br /> <br />The river system consists of several 30 to 70 mile sections of <br />relatively homogeneous fishery habitats, e.g., high-gradient canyons, <br />low-gradient braided winding sections and transition zones between <br />these two main types. Our sampling scheme identified these homogeneous <br />sections and handled them as discrete strata of a random sampling <br />scheme to provide a good distribution of samples and to reduce within- <br />strata included both geophysical (river channel configuration, substrate <br />types, gradient and depth) and biological parameters (productive <br />bottom lands, sterile sand runs, productive backwaters, swift canyons <br />and point sources of allochthonous materials). <br /> <br />Because we could work sequentially downstream with less investment <br />of time, we did not use a completely random design to select the <br />sequence of strata to be sampled; but instead used a systematic sampling <br />design with a random start. Utilizing a table of random digits, one <br />stratum was selected as the starting point with the sampling series <br />progressing sequentially downstream to the last strata, shifting back <br />to the uppermost stratum to complete the series. <br /> <br />Within each stratum a sample site was selected according to a <br />completely random sampling design. Each stratum was divided into <br />river-mile stations. A table of random digits was consulted and the <br />first numbers encountered in the table that appeared in the section <br />were chosen as the sample station. To permit replication and to <br />extend the boundaries of each sample, each station consisted of two <br />0.5 mile reaches; one extending downstream from the selected river <br />mile starting point, the other five-miles downstream (Figure 1). <br />Intensive sampling began in the uppermost 0.5 mile section continuing <br />for 24 hours. The second day, the intervening 4.5 mile section was <br />surveyed by electrofishing starting at the lower boundary of the <br />previous sample site and continuing downstream to the upstream point <br />of the second 0.5 intensive section. From this point, the second 0.5 <br />mile section was intensively sampled for 24 hours. Generally, sampling <br />was discontinued after the second intensively sampled reach was comp1ete( <br />and the field crew usually returned to home station. <br /> <br />In some instances, it was advantageous to sample two reaches by <br />proceeding immediately from one to another. There were also times <br />that equipment failure dictated minor schedule changes. No change was <br />allowed unless compatihle with the philosophy of the sampling design. <br /> <br />The intent of the sampling procedure as outlined was to provide <br />a basis for the collection of both positive and negative correlations <br />hetween habitat and endangered fish species by sampling all river <br />