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<br />damage (e.g., ruptured chorion) and measured (maximum diameter) under a <br />stereo-zoom dissecting microscope. After stripping, fish were immediately <br />euthanized with an overdose of tricaine methanesulfonate (MS-222), labeled as to <br />treatment, and placed in an ultra-cold freezer at -840C for later internal <br />examinations. Fertilized eggs from each treatment replicate or the control were <br />placed in separate plastic bags, bags were inflated with pressurized O2 and sealed, <br />and eggs were transported to Colorado State University, Fort Collins. <br /> <br />In the laboratory, a sample of eggs from each treatment replicate or the <br />control was divided into 5 (shocked replicates) or 10 lots (contro!), each with 500 <br />eggs. Each lot of eggs was placed in a separate incubation basket that was <br />randomly positioned in a flow-through trough receiving well water at 180C. Eggs <br />were examined at least twice daily during incubation, and obviously dead eggs in <br />each lot were recorded and removed to reduce spread of fungus. Time of hatching <br />(hours post-fertilization) and hatching success were recorded for each lot of eggs. <br />Weighted analysis of variance was used to compare mean egg hatching success <br />among treatments and the control because of unequal number of egg lots. Fisher's <br />pair-wise comparison test was used to identify significant differences (P ~ 0.05) <br />between individual means. <br /> <br />For internal examination of adults, fish were partially thawed, X-rayed dorsally <br />and laterally (left side) with a portable MinXray@ model X750-G on 3M@ medical <br />imaging film (35 X 43 cm), and necropsied. X-ray plates were inspected for <br />evidence of damage to vertebrae. Necropsy consisted of filleting fish along both <br />sides of the spine and then examining the spinal column and surrounding <br />musculature for evidence of hemorrhages. Fish were also opened along the <br />mid-ventral line, from vent to head, and examined for evidence of damage to internal <br />organs. Type and severity of injuries associated with the spinal column were <br />classified according to criteria proposed by J. B. Reynolds (Alaska Cooperative Fish <br />and Wildlife Research Unit) as presented in Hollender and Carline (1994). Vertebral <br />injuries were classified as: compression of vertebrae (class 1), misalignment and <br />compression of vertebrae (class 2), or fracture of ~ 1 vertebrae or complete <br />separation of ~ 2 vertebrae (class 3). Hemorrhages were classified as: wounds <br />separate from spine (class 1), wounds on spine::;; width of 2 vertebrae (class 2), or <br />wounds on spine> width of 2 vertebrae (class 3). <br /> <br />Results <br /> <br />Tetany was induced in all shocked fish during treatment, but those exposed to <br />CPS continued to quiver after they appeared to be tetanized, whereas fish subjected <br />to the 50-Hz current did not. All males and females exposed to either current <br /> <br />7 <br />