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Last modified
7/14/2009 5:02:32 PM
Creation date
5/22/2009 4:56:17 PM
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UCREFRP
UCREFRP Catalog Number
7959
Author
Muth, R. T. and J. B. Ruppert.
Title
Effects of Two Electrofishing Currents on Captive Ripe Razorback Sucker and Subsequent Egg Hatching Success - Final Report.
USFW Year
1995.
USFW - Doc Type
(CO River Recovery Program Project No. 61 RMD/9595),
Copyright Material
NO
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<br />pulsed DC by a Coffelt@ WP-15 or Coffelt Mark 20 Complex Pulse System (CPS TM) <br />electrofishing control unit. Settings for the WP-15 were 60 Hz and 24% duty cycle <br />(4-ms pulses). These settings a:-e commonly used with the WP-15 in boat <br />electrofishing for adult razorback sucker in the middle Green River system (E. Wick, <br />U.S. National Park Service, personal communication). The CPS unit, a newer and <br />potentially less harmful control unit (Fredenburg 1992; Sharber et al. 1994), <br />produces a fixed complex-pulse pattern consisting of 15-Hz "packets" of three <br />240-Hz, 2.6-ms pulses which results in a 12% duty cycle. Each control unit was set <br />at 200 V, and output was measured at 0.45 A. Typical output from a WP-15 during <br />electrofishing on the Green and Colorado rivers, Utah, at conductivities of 300-600 <br />pS/cm is 200-300 V and 5-7 A (T. Chart, Utah Division of Wildlife Resources, <br />personal communication). <br /> <br />The treatment chamber was a 227 -cm long X 53-cm wide X 53-cm deep <br />fiberglass tank with full cross-sectional, steel-plate electrodes placed parallel 191 cm <br />apart to provide homogeneous electric fields with a peak-voltage gradient of 1.0 <br />V/cm as measured by an oscilloscope. According to data summarized by Snyder <br />(1992), a peak-voltage gradient of 1.0 V/cm should be sufficient to induce at least <br />narcosis in many fishes. Water in the tank was 47 cm deep, 20oC, and had a <br />conductivity of 610 pS/cm which approximated mean conductivity in the Green River <br />near razorback sucker spawning areas upstream of Jensen, Utah, during late April <br />through May I the period when peak spawning of razorback sucker usually occurs (T. <br />Modde, U.S. Fish and Wildlife Service, personal communication). A plastic-mesh <br />holding basket, 90 cm long X 38 cm wide X 37 cm deep, was suspended midway <br />between the electrodes to confine fish during treatment and keep them away from <br />the electrodes and treatment-chamber walls and bottom. <br /> <br />Treatment with either current was replicated twice, and each replicate <br />consisted of two males and two females, except for one replicate subjected to CPS <br />that consisted of two males and one female. There was one control consisting of <br />two males and two females. Each group of fish was randomly assigned to <br />treatments or control. Control fish were handled the same as shocked fish but <br />without exposure to electric current. Fish in each treatment replicate were placed <br />one at a time in the holding basket with their head toward the anode and subjected <br />to either current for 10 s, an exposure time used in other investigations (e.g., Dwyer <br />and White 1995) and that might be at least occasionally expected when <br />electrofishing. Observations were made on fish behavior and condition before and <br />during treatment. Immediately before and after treatment, each fish was examined . <br />for external evidence of hemorrhaging (bruises or brands). <br /> <br />Gametes were manually stripped from fish in each treatment replicate or the <br />control and combined for egg fertilization. Samples of eggs were taken before <br />fertilization and immediately after water hardening and preserved in 10% formalin. <br />Subsamples of these eggs (N = 50) were later examined for evidence of obvious <br /> <br />6 <br />
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