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<br />6 <br /> <br />This procedure resulted in a sampling of far more sites per field trip <br />than determined by the random selection process alone. Seine samples were <br />taken with 3.0- x 1.2-m and 1.0- x 1.2-m seines (1.6-mm square mesh). <br />Samples were collected according to a habitat stratification scheme designed <br />to reflect the geomorphic, hydrologic, and ecologic variables of the study <br />area. Habitat variables were described in detail in Haynes and Muth (1982). <br />The width and length of each seine collection were measured with a metric <br />tape and area sampled (m2) was calculated and recorded. Larger specimens, <br />identifiable to species, were measured to the nearest 0.1 mm (total length, <br />TL), counted, and released. Smaller specimens were preserved in 10% buffered <br />formalin and returned to the Larval Fish Laboratory (Colorado State <br />University, Fort Collins) for processing. <br /> <br />An index of relative young-of-the-year (YOY) abundance (i.e., capture per <br />unit effort, C/f) was developed for seine-collected YOY Colorado squawfish <br />using basic tenets of Ricker (1975), Caughley (1978), Lackey and Hubert <br />(1978), Southwood (1978), and Tanner (1978), i.e., that C/f is related in a <br />constant or predictable way to population size and changes in absolute <br />abundance over space and time will be reflected by changes in C/f. A <br />discussion of the advantages and disadvantages of using C/f methods in open, <br />structurally diverse systems such as the Upper Colorado River Basin was <br />presented in Haynes and Muth (1984). C/f methods were selected in favor of <br />other, more rigorous, abundance models because closure of the system was not <br />possible. C/f values (numbers of YOY per 100 m2) were derived from seine <br />samples of known area and specific habitat features. An evaluation of YOY <br />Colorado squawfish distribution per specific habitats was made for 1981-1984 <br />for each river and only those habitat types in which YOY had been previously <br />collected were used for C/f calculations (i.e., backwaters, embayments, <br />concavities, pools, isolated pools, and shallow shorelines). In this way, <br />habitats such as riffles, which had never yielded YOY Colorado squawfish, <br />were omitted from the calculations. Further, for any given year and river, <br />samples taken prior to the earliest estimated spawning dates (derived from <br />age estimates of YOY, presented later in Methods) were also excluded. In the <br />Yampa River, only seine samples taken at or below river km 32.0 (the <br />previously suspected upstream limit of Colorado squawfish spawning in the <br />Yampa River) were included in C/f calculations. <br /> <br />During July-August 1982, icythyoplankton drift-nets were deployed in the <br />Black Rocks area, Colorado River, to determine if larval Colorado squawfish <br />disperse via dOliUstream drift and to evaluate gear, sampling design, and <br />methods of gear deployment. These initial trials proved successful and it <br />was decided to extensively utilize drift-nets in the Yampa River (June-August <br />1983; July-August 1984) and Green River (Ju1y--August 1984). Drift-nets were <br />deployed at two Yampa River sites in 1983 (i.e., Harding Hole, river km 32.5; <br />and Box Elder, river km 3.1). The Harding Hole site was selected to evaluate <br />the possibility that Colorado squawfish spawn above river km 32.0. The Box <br />Elder site was chosen to assess larval fish drift patterns near the Yampa <br />River mouth. In 1984 drift collections continued at Box Elder, while the <br />Harding Hole site was dropped in favor of a Green River site approximately <br />9.3 km below the Yampa-Green River confluence (river km 355.3, Colorado-Utah <br />state line). This site was included to follow progression of drifting fish <br />larvae down the Green River. <br />