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<br />7 <br /> <br />Near-shore drift collections were made using 0.5-m diameter conical <br />plankton nets (Wildlife Supply Company, Saginaw, Michigan) mounted on 0.5- x <br />0.3-m rectangular steel frames and fitted with 33-cm long (lO-cm diameter) <br />removable PVC collection buckets with a threaded cod end. Each net had a <br />560-micron Nytex nylon mesh length of 4.0 m, and an open mesh to net mouth <br />ratio of 11:1. Filtration efficiency approaches 100% when the open mesh area <br />is more than three times the area of the net mouth (Faber 1968; Tranter and <br />Smith 1968). In the Colorado River, a removable four-point, steel-cable <br />bridle assembly terminating in a spring-loaded carabiner was attached to each <br />net frame. Nets were deployed either by staking the net frames to the river <br />substrate in shallow areas or fastening the bridle carabiner to a <br />polypropylene line fixed to either an instream boulder or a metal post driven <br />into the shore at deeper sites. Mid-channel sets were attempted but <br />discontinued for safety reasons and because of possible boat traffic during <br />collection times. In the Yampa River, drift-nets were deployed off rafts <br />tied to shore during runoff periods when depths and currents made wading <br />difficult. During lower water periods, nets were attached directly to metal <br />posts driven into the river substrate. At all sets, a safety line was <br />attached to each net frame and to a post driven into the shore. <br /> <br />Three drift-nets were deployed along the shoreline at each site just <br />below the water surface. Water volume passing through each net was <br />calculated from velocity readings made with either a Marsh-McBirney (Model <br />201) or pygmy (Gurley Model 625 F) current meter. Water temperature (C) was <br />measured at each sampling time. In order to evaluate possible diel <br />periodicity in larval fish drift, samples were taken at sunrise, noon, <br />one-half hour after sunset, and at midnight. Sampling duration at each <br />period ranged from 1 to 2 hours depending upon the suspended solids load. <br />The contents of each net were rinsed into l-gallon plastic jugs, preserved in <br />10% buffered formalin, and returned to the Larval Fish Laboratory for <br />processing. All specimens were counted, measured to the nearest 0.1 mm (TL), <br />and assigned to a developmental phase according to Snyder (1976). This <br />procedure permitted evaluation of larval fish drift periodicity both within a <br />particular developmental phase (e.g., protolarval) and between phases (e.g., <br />protolarval vs mes~larval). Numerical densities were computed as numbers of <br />larvae per 1,000 m. Two-way analysis of variance (2-ANOVA) was used to <br />compare drift densities (response variable) among the four sampling times and <br />between day and night samples (i.e., combined sunrise and noon = day; <br />combined sunset and midnight = night) on individual sampling dates over the <br />entire sampling season. The 0.05 probability level was used to determine <br />significance in F values. <br /> <br />Flow and temperature data were obtained from United States Geological <br />Survey (USGS) readings when available and supplemented with measurements <br />taken during sampling and CRFP thermograph data. All field and laboratory <br />data were recorded to be compatible with data collected by NW Region (CDOW) <br />and CRFP personnel with any differences resulting from specific requirements <br />of early life history studies. Data were generally compatible with the <br />United States Fish and Wildlife Service MANAGE database program. All field <br />data has been filed on dBase II and stored at CDOW, Denver. <br /> <br />To estimate spawning dates using back-calculated age of seine- and <br />drift-net-collected young-of-the-year (YOy) Colorado squawfish, two <br />predictive age equations (Haynes and Muth 1984) were derived using Hamman's <br />(1981) total length at known age data for hatchery cultured and reared <br />Colorado squawfish larvae (Fig. 2). <br />