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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />7 <br /> <br />Research on the Colorado squawfish intensified after <br />the fish was listed as an endangered species in 1973. It <br />has focused largely on life history and ecology (Seethaler <br />1978; Tyus 1986), spawning behavior (Hamman 1981; Lamarra et <br />ale 1985; Tyus et ale 1981; Tyus 1985), habitat requirements <br />(Tyus and McAda 1984), preferred and optimal temperatures <br />(Black and Bulkley 1985a, 1985b), and food habits (McAda and <br />Tyus 1984; Pimentel et ale 1985). Studies of overwinter <br />survival of age-O Colorado squawfish are conspicuously <br />lacking. Osmundson (1987) found no evidence of size~ <br />selective overwinter mortality among hatchery-reared <br />Colorado squawfish stocked into ponds near Grand Junction, <br />Colorado. However, the smallest fish at the onset of winter <br />in Osmundson's experiment were already 54 mm total length <br />(TL) , which is significantly larger than the mean length of <br />wild Colorado squawfish at the same time of year (Tables 1 <br />and 2). As part of an investigation preliminary to the <br />present study, I held 331 hatchery-reared age-O Colorado <br />squawfish 39 to 87 mm TL, at simulated winter temperatures <br />from 22 January 1988 to 8 July 1988 (Appendix H). Fish were <br />fed pelleted trout food twice weekly at 2% of the total fish <br />biomass in each aquarium. survival rate was 100% for all <br />sizes of fish during the 150-day winter period. The <br />majority of the fish used in this preliminary effort, <br />however, were too large to be representative of the sizes of <br />age-O Colorado squawfish in nature (Tables 1 and 2). In <br />