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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />METHODS AND MATERIALS <br /> <br />Study area <br /> <br />Three study areas with previously reported differing populations of <br />larval Colorado squawfish were chosen. These three areas were roughly <br />one-mile in length and were located at Island Park in Dinosaur National <br />Monument, at Jensen downstream from the Route U.S. 40 bridge over the <br />Green River, and at Ouray on the Ouray National Wildlife Refuge (fig. 1). <br />Previous studies by the USFWS indicated increasing abundance of larval <br />Colorado squawfish downstream from Island Park to Ouray. Abundance <br />of larval Colorado squawfish is apparently greatest in the Ouray area. <br /> <br />In 1986, USFWS personnel designated certain backwaters that apparently <br />persisted from year to year as "reference" backwaters. In 1987, the <br />"reference" backwater at Island Park did not develop, however, so a <br />main river site and two backwaters were selected upstream near the <br />confluence of Garden Creek (BA-332.2) and at Big Island (BA-333.2). <br />The Garden Creek backwater was initially large but was greatly reduced <br />in size as river flows decreased during the summer and essentially <br />was the "small" backwater for comparison at Island Park. Garden Creek <br />backwater is at RMI (River Mile Index) 332.2. Initially the small <br />backwater was a backwater off a side channel of the river; this backwater <br />dried up as riverflows dropped, cutting off the side channel and <br /> <br />4 <br />