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NEST-SPECIFIC BASS DNA FINGERPRINTS 453 2 3 4 5 7 6 61 kb- .. ~ . ... ... ~-,:,,;,-.........d tiLl ... . . r . 1 ... f . -- ..' IJ '" . . --- . . ~ . . .. . ...~ . .- . 30 kb - 16kb- FIGURE 3,- The DNA fingerprints of seven small mouth bass fry collected from nest C32 in Lake Opeongo (probe [GGA TJ4 and enzyme Hae III), vidual to its nest of origin and assess if nest-spe- cific fingerprints exist. Eight fry that had not been fingerprinted previously were chosen by a second party and their vials were arbitrarily relabeled; the second party kept a hidden list of the nest of origin of each vial. Fingerprints were generated for all fry and their banding patterns (distribution and intensity of bands) were compared visually to each of the 15 nest fingerprints to assign a nest of origin, A match was determined when the banding pat- tern of a fry concurred with the overall banding pattern of a particular nest fingerprint. Assign- ments were compared with the list of actual nest origins for an assessment of the ability of this tech- nique to correctly identify nest origin, Results The best DNA fingerprint patterns were ob- tained with the restriction enzyme Hae III and the oligonucleotide probe (GACAk Compared with the other combinations of enzymes and probes, the banding pattern generated by Hae III and (GACA)4 consisted ofa moderate number of bands per individual (36 i: 4, mean i: SD) that migrated throughout the 1.6-14-kb range (Figure 2), This combination of enzyme and probe produced a low level of polymorphic loci across members within a nest, as was evident from the average SI of87,2 :t 4.4 (Table I), Similar levels of polymorphism TABLE I.-Average similarity indices of DNA finger- prints and numbers of comparisons between individual smallmouth bass for three categories of fry or guardian males, Spatial rela1ionships of individual nests and clus- ters of nes'ts are shown in Figure \, Comparisons between Fry within a nest Fry from several nests closely spaced together in clusters Fry or guardian males from different nests within Jones Bay Number Similarity of com- index pansons (mean :t SO) 62 87,2 :t 4.4 13 67,2 :t 6,0 15 52,5 :t 5,8