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<br />GROSS ET AL.
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<br />FIGURE 2,- The DNA fingerprints of eight smallmouth bass fry (lanes 1-8) and the guardian male (M) collected
<br />from nest C31 in Lake Opeongo (probe [GACA]4 and restriction enzyme Hae III ), Each numbered lane contains
<br />the DNA from an individual fry; the DNA has been cui into fragments with the enzyme and eiectrophoretically
<br />separated out by size through an agarose gel. Each band, ranging in size from approximately 1.5 to 13 kilobases
<br />(kb), is the result of a radioactively labeled probe pairing with the complementary sequence in a particular DNA
<br />fragment, which then has been visualized by exposure to X.ray film,
<br />
<br />of one lane of I-kb marker (Gibco BRL), eight
<br />lanes each containing the DNA of an individual
<br />fry from the nest, and one lane containing the
<br />DNA of the guardian male of the nest.
<br />Again, with the same combination of enzyme
<br />and probe as above, two gels were run to examine
<br />the differences in fingerprints between nests in
<br />Jones Bay. One gel consisted of one fry randomly
<br />chosen from each of 10 nests and the other con-
<br />sisted of 10 guardian males, In addition, several
<br />gels were run to compare fingerprints of fry from
<br />nests that were spaced closely together in clusters
<br />(Figure I),
<br />A similarity index (SI) was computed to measure
<br />genetic variability, The SI between two individ-
<br />uals was calculated by the formula 100' 2Nxy/(Nx
<br />
<br />+ Ny); Nxy is the number of bands shared be-
<br />tween individuals X and Y. and Nx and Ny are
<br />the total number of bands scored for X and Y.
<br />respecti vely (Lynch 1990), Comparisons of bands
<br />were made between individuals in adjacent lanes
<br />down to approximately the 1,6-kb fragment. Al-
<br />though bands were clearly distinguishable below
<br />1,6 kb, they were not included in the analysis be-
<br />cause they might upwardly bias the SI estimates
<br />due to a higher probability that these bands are
<br />shared (Jeffreys et al. 1985), For all gels, the same
<br />investigator scored bands as identical if they had
<br />similar mobility and intensity based on visual es-
<br />timation,
<br />A double-blind test was conducted to determine
<br />the ability to correctly assign an unknown indi-
<br />
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