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<br />National Park Service, personal communication). A fixed complex-pulse current was <br />delivered by the CPS unit, a newer and potentially less harmful control unit (Fredenburg <br />1992; Sharber et al. 1994). The CPS pulse pattern has a duty cycle of about 12% and <br />consists of 15 "packets" or "trains" per second of three 240-Hz, 2.6-ms pulses (each <br />separated by approximately 1.6 ms). Each control unit for all currents was set at 200 V <br />output. Typical output from a WP-15 during electrofishing on the Green and Colorado <br />rivers, Utah, at conductivities of 300-600 pS/cm is 200-300 V and 5-7 A (T. Chart, Utah <br />Division of Wildlife Resources, personal communication). <br />A fiberglass tank, 305 cm long X 30 cm wide X 30 cm deep, filled with well water at <br />18.50C was the treatment chamber. Water in the tank was 24 cm deep and had a <br />conductivity of 650 pS/cm which approximated mean conductivity in the Green River near <br />razorback sucker spawning areas upstream of Jensen, Utah, during late April through May, <br />the period when peak spawning of razorback sucker usually occurs (T. Modde, U.S. Fish <br />and Wildlife Service, personal communication). Electrodes were two 30 X 30-cm pieces of <br />sheet metal placed parallel 21-173 cm apart to cover the full cross-sectional area of the <br />treatment chamber and prOVide homogeneous electric fields. Distance between the <br />electrodes was adjusted to produce the desired peak-voltage gradient. Peak-voltage <br />gradients, as measured with an oscilloscope, were 1.2, 5.0, or 10.0 V/cm for the 60-Hz <br />current and 1.2 V/cm for the 30-Hz, 80-Hz, and CPS currents. Corresponding power <br />densities (Koltz 1989) were calculated as 936 pW/cm3 (1.2 V/cm), 16,250 pW/cm3 (5.0 <br />V/cm) , and 65,000 pW/cm3 (10.0 V/cm). Treatments at 5.0 and 10.0 V/cm with the <br />commonly used 60-Hz current were included to evaluate response of embryos and larvae to <br />increases in electric-field strength. Peak voltage in the water for all electric fields was <br />measured at 208 V. <br />Viable embryos at one of the three developmental stages and pre-swimup larvae <br />were exposed to one of the six electric fields for 10 s, an exposure time used in other <br />investigations on effects of electrofishing fields on fish embryos (Dwyer et al. 1993; Dwyer <br />and Erdahl 1995). Each treatment and corresponding control was replicated three times. <br />Each replicate consisted of 20 embryos or larvae and was randomly assigned to treatments <br />or controls. For shocking, embryos or larvae in each replicate were placed in a separate <br />16-cm long X 12-cm wide X 13-cm deep nylon-mesh basket suspended midway between <br />the electrodes. Controls were handled the same as treatments but without exposure to <br />electric current. Observations were made on behavior of larvae before and during <br />treatment. <br />For continued incubation or rearing after treatment, eggs were kept in the nylon- <br />mesh baskets and larvae were transferred to 1-L glass beakers containing aerated well <br />water. Containers were randomly placed in flow-through troughs, each 300 cm long X 30 <br />cm wide X 20 cm deep, receiving well water at 180C. Eggs were examined at least twice <br />daily through end of hatching, and obviously dead eggs in each replicate were recorded and <br />removed to reduce spread of fungus. Time of hatching (hours postfertilization) and number <br />of hatched larvae were recorded for each group of eggs. Hatched larvae were euthanized <br />with an overdose of tricaine methanesulfonate (MS-222), fixed and preserved in 3% <br />phosphate-buffered formalin, and later examined for evidence of obvious external <br />abnormalities. Larvae treated at pre-swimup were reared for 4 weeks after treatment, and <br />each replicate received as food the same measured quantity of recently hatched nauplii of <br />Artemia sp. twice daily. Nauplii not consumed within 2 h after each feeding were siphoned <br />from the beakers. Immediately after treatment and at weekly intervals thereafter, a random <br />sample of seven larvae from each replicate was anesthetized in a solution of 100 mg/L <br /> <br />7 <br />