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<br />12 BIOLOGICAL REPoRT 19 <br /> <br />build in some replication; however, the time and <br />cost were prohibitive. <br /> <br />Level N <br /> <br />This component involves using infrared aerial <br />photography on the same 10 watersheds examined <br />in Level II. Aerial infrared photos were taken in <br />spring 1992 before leaves were present. Ground <br />photos were taken concurrently to aid in aerial <br />photo interpretation. A mosaic of all aerial photos <br />will be made; landscape features will be identified, <br />mapped on mylar sheets, and recorded in digitized <br />fonn. We hope this level of assessment will prove <br />as reliable as and more economical than Levels II <br />and IlL <br /> <br />Biological Monitoring <br />Subcommittee <br /> <br />Sediment Chemistry <br /> <br />During the last 15 years, total suspended solids <br />in the lower Minnesota River have been six times <br />greater than those in the Mississippi River at their <br />confluence (MPCAfiles). Contaminants such as <br />heavy metals and PCB's often associate with <br />smaller particles in suspended solids and sedi- <br />ments (Oliver 1989). <br />Suspended solids, sediments, sediment pore <br />water, and mussels were collected at four main- <br />stem and five tributary sites once during spring <br />and once during summer 1990. Suspended solids <br />were analyzed for pH, percent organic matter, <br />nickel, cadmium, lead, copper, zinc, and chro- <br />mium. Zinc was the most abundant of the heavy <br />metals found, and cadmium was the least abun- <br />dant. <br /> <br />Ambient Toxicity <br /> <br />Toxicity tests frequently show good correlation <br />with elevated instream pollutants and down- <br />stream persistence (Ankley et al. 1990). The objec- <br />tive of this component of the study is to determine <br />if ambient toxicity can be demonstrated in Minne- <br />sota River surface water and sediments. Thirteen <br />mainstem sites, eight tributary sites, and four <br />reservoir sites were selected. These sites were cho- <br />sen based on the following criteria: good road ac- <br />cessibility and availability of stream sediment, <br />area to launch a small boat, and proximity to <br />physical and chemical subcommittee monitoring <br />sites. <br /> <br />Ambient water and sediment samples were col- <br />lected in midchannel to avoid shoreline distur- <br />bances. Samples were collected during five periods <br />in 1989 and 1990 to cover all four seasons and to <br />coincide with other sampling activities. Anion and <br />cation concentrations were determined for each of <br />the samples. <br />Two standardized test procedures were applied <br />to the surface water and sediment pore water. <br />Ceriodaphnia, a microcrustacean. and Selanas- <br />trom, a green alga, were the test organisms. Ceri- <br />odaphnia test procedures are described by Horn- <br />ing and Weber (1985), and Selanastrom test <br />procedures are described by the U.S. Environ- <br />mental Protection Agency (1989). <br />Toxicity to Ceriodaphnia was found at 5 of the <br />25 sampled sites. The most highly toxic sites were <br />found near the mouth of the Minnesota River and <br />in Lac Qui Parle Reservoir. Mainstem locations <br />upstream from rkm. 17 and the eight tributary <br />locations were nontoxic, but three of the four res- <br />ervoir sites were toxic. <br />Toxicity to Selanastrom was found at 8 of the 15 <br />sampled locations. Toxicity was found at all sites <br />in the mainstem between rkm. 336 and the mouth <br />and at both sampled reservoir sites. None of the <br />five sampled tributaries was toxic. The most toxic <br />site to Selanastrom was found in Lac Qui Parle <br />Reservoir. <br />Reservoir sites had higher concentrations of to.. <br />tal nutrients in sediment pore water, mainstem <br />sites showed higher concentrations of nitrite, and <br />tributary sites had higher concentrations of ni- <br />trate. Four locations-two mainstem (Fort <br />Snelling and I -35W Bridge) and two reservoirs <br />(Rapidan and Lac Qui Parle)-had the highest <br />concentrations of ammonia and total phosphate. <br />Three mainstem locations (I-35W Bridge, Judson, <br />and Courtland) had nitrite levels more than 100 <br />times higher than the other sites. Four tributary <br />locations (Le Sueur, Blue Earth, Watonwan, and <br />Rush rivers) had sediment nitrate concentrations <br />2-10 times greater than the other locations. Am- <br />monia, nitrate, total phosphate, and iron showed a <br />progressive increase in downstream mainstem lo- <br />cations. <br /> <br />Algal Communities <br /> <br />The composition and abundance of algal com- <br />munities in streams reflect characteristics of the <br />sUlTOunding watershed, riparian zone, and chan- <br />nel morphology. These features influence the <br />physicochemical conditions of water and substrate <br />