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<br />Severson et al. <br /> <br />59 <br /> <br />over the remaining diets (AP-I00 and Dio-dry) because of its si~i- <br />lar performance to them, cost, availability, and its widespread use <br />in coldwater hatcheries. On day 71, fish fed A-250 were switched <br />to A-400, a 400-Jj. granulation of the same formulation. The fish <br />were hand-fed six times per day for a daily ration of 10% of total <br />body weight. Feeding rate was adjusted at two-week intervals. <br />Survival and growth was calculated at the end of each experimen- <br />tal phase. Instantaneous daily growth (0) was calculated from ini- <br />tial weight and final weight for each diet: <br /> <br />o = (lo&: W2 - lo&: w,)/d <br /> <br />where w, and W2 are the initial and final average weights, respec- <br />tively, and d is the duration of study in days (Ricker 1975). One- <br />way analysis of variance and Tukey multiple comparison tests were <br />used to determine differences in fish growth and sUlvival (Sakal and <br />Rohlf 1981) in Phase II. An arcsine transformation was applied to <br />survival rates to more closely approximate normality. Statistical <br />tests were not performed on Phase III because only one of the four <br />treatments had replicat~s. <br />At the end of Phase III, the fish were measured and examined for <br />gross deformities, and five fish from each experimental lot were <br />sent to the Bozeman Fish Technology Center, Bozeman, Montana <br />for histological examination. <br /> <br />RESULTS AND DISCUSSION <br /> <br />~ <br />".....-..:':'.,":........ ; <br /> <br />Phase I (Day 5-25) <br /> <br />- Survival was 96% (N = 3,359) for razorback sucker fry fed the <br />B-250 diet for 20 days. Average fish weight increased from 0.005 g <br />to 0.01 g (SD = 0.022), a daily weight gain of about 4% (0 = <br />0.038). These results were consistent with results from an earlier <br />study (Tyus and Severson 1990) in which razorback sucker fry fed <br />B-250 for 45 days had a survival rate of 78% and a daily weight <br />gain of about 5% (0 = 0.046). The higher survival in this study <br />was presumably because daily feeding rate was greater. <br />