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Surgical Procedure <br />Each radio transmitter was dipped in melted beeswax to cover any <br />abrasive surfaces which might irritate the fish. Frequency and pulse <br />rate of the transmitter were checked after dipping and before insertion. <br />The fish to be tagged was placed in 'a large tub containing 150-250 mg/L <br />of tricain methane sulfonate (MS-222) and watched until opercle movement <br />stopped. The fish was then placed on a measuring board that was washed <br />with MS-222 solution. During surgery the fish's gills were also irri- <br />gated with MS-222. Operating instruments were sterilized with isopropyl <br />alcohol after boiling. <br />A short 3-4 cm incision was made with a number 15 scalpel blade in <br />the side of the fish just anterior to the pelvic fin and ventral to the <br />rib cage. The radio tag was inserted into the body cavity with the <br />antennae loop toward the head of the fish. The incision was then closed <br />with a monofilament nylon 000 suture with a FS-1 cutting needle. Five <br />individual sutures were tied with surgeon's knots and each knot was <br />sealed with "super glue" and inspected for tightness. <br />After the incision was closed the fish was held in the quiet water <br />of Gypsum Bay until it recovered from the anesthetic. Recovery was rapid <br />(3-4 minutes) and the fish was released immediately into the bay. <br />RESULTS <br />A single squawfish large enough for surgical implant (817 mm, 4,082 <br />grams) was captured by gillnet on June 9 at 10:00 a.m. in a back eddy 3-4 <br />meters deep near river mile 196. The fish was in the net for less than <br />30 minutes and was in excellent condition both before and after surgery. <br />The fish was released after tag insertion and tracked in Gypsum Canyon <br />where it moved around the bay during the daytime on June 9. On June 10 <br />extensive tracking failed to locate the fish in Gypsum Canyon. Boats <br />tracked upstream to the Cataract Canyon rapids and downstream approxi- <br />mately 5 km without receiving a signal. This search procedure was <br />repeated on the following day (June 11). <br />On June 12 two boats tracked from Gypsum Canyon to Hite Marina <br />(river mile 168). Four signals were received with the tracking receiver <br />(RF-40) near Rock Canyon (river mile 173) and then the signal was lost. <br />Repeated attempts to relocate the fish failed. Water was approximately <br />37 meters (120 ft) deep at Rock Canyon; if the fish sounded deep, range <br />of the signal was probably too low to permit detection. Tracking was <br />continued on alternate days through the month of June and into early July <br />without locating the tagged fish. <br />Several theories have been presented to explain the relatively high <br />capture rate of this endangered species at the Colorado River/Lake Powell <br />mixing zone during the spring of 1980. One theory suggests that the <br />squawfish captured during 1980 were residents of the reservoir and moved <br />upstream to the headwaters, perhaps on a spawning migration. The high <br />condition factor (KTL of many fish captured in 1980 suggested they were <br />277