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to 27 C are about 2 C lower than measured by Deacon et al. (1987) for <br />speckled dace from the Virgin River in Utah acclimated to 25 C. This <br />difference may be related in part to the much larger specimens (average of <br />72.2 mm) used in that study. Speckled dace used in the present trials were <br />deliberately selected for small size so that they would be similar to the <br />Kendall Warm Springs dace. however, they were still significantly larger. <br />Electrophoretic Studies <br />The 26 loci examined for 12 specimens each from Kendall Warm Springs <br />and Duck Creek are listed in Table 3. No differences were discernable at <br />Table 3. Enzymes examined for comparisons of R. o_ thermalis <br />and R. o_ yarrowi. <br />Locus <br />Enzyme <br />Buffer* <br />AAT-1,2 Aspartate aminotransferase R <br />AGP Alpha-glycerophosphate dehydrogenase 4 <br />EST-1,2 Esterase R <br />GAPDH Glyceraldehyde-3-phosphate dehydrogenase C <br />GDH Glutamic dehydrogenase C <br />GP-1.2,3 General protein C <br />GPI-1,2 Giucosephosphate isomerase C <br />G6POH Glucose-6-phosphate dehydrogenase R <br />HBDH Hydroxybuteric dehydrogenase C <br />IOH-1,2 Isocitrate dehydrogenase C <br />LDH Lactate dehydrogenase 4 <br />MDH-1,2 Malate dehydrogenase C <br />ME Malic enzyme R <br />MPI Mannosephosphate dehydrogenase R <br />PGD Phosphogluconate dehydrogenase 4 <br />PGM Phosphoglucomutase C <br />S00-1,2 Superoxide dismutase R <br />XDH Xanthine dehydrogenase C <br />* R - Ridgeway, G. J. et al., 1970. Trans. Am. Fish. Soc. 99:147-151; <br />4 - Selander, R. K. et al., 1971. Univ. Texas Publ. 7103:49-90; <br />C e Clayton, J. W. and D. N. Tretiak 1972. J. Fish Res. Bd. Can. <br />29:1169-1172. <br />these loci between Kendall Warm Springs and speckled dace. No variants <br />were observed at any locus in either population, and both populations were <br />fixed for the same eiectromorphs at all loci. <br />Mitochondrial DNA Studies <br />Initial results from four Kendall Warm Springs dace and seven speckled <br />dace from Duck Creek are depicted in Fig. 1. Five of the eight restriction <br />enzymes reveal polymorphic mtDNA in fish from Duck Creek, and one enzyme <br />revealed a polymorphic mtDNA in fish from Kendall Warm Springs. On the <br />basis of this small sample analyzed to date, Kendall Warm Springs dace <br />appear to have inherited only one form of the mtDNA found in speckled dace <br />in this nearby tributary of the Green River. <br />Captive Breeding and Rearing <br />Speckled dace have spawned repeatedly in our laboratory tanks, but <br />8