9586

• UCREFRP Catalog Number: 9586
• Author: Desert Fishes Council.
• Title: Proceedings of the Desert Fishes Council Volume XXVI 1994 Annual Symposium.
• USFW Year: 1994.
• USFW - Doc Type: Furnace Creek, CA.
• Copyright Material: NO

PROCEEDINGS OF THE DESERT FISHES COUNCIL, 1994 SYMPOSIUM VOLUME XXVI - published 1995
y/o el Lago Powell y/o el Lago Mohave. Recomendamos no usar los pocos matalotes remanentes de la poblaci6n
del Alto Colorado en un programa de reproducci6n, por su reducida variati6n producto de la deriva gen6tica, to
cual tambi6n puede fortalecer su proporci6n de genes no deseados como producto de la hibridaci6n con el matalote
boca de franela (flannelmouth sucker)
DRML,S.L. (Department of Biology, University of California, Los Angeles, Los Angeles, CA)
A genetic analysis of artificial propagation of the endangered razorback sucker, Xyrauchen texanus
Analisis gen6tico de propagaci6n artificial del matalote jorobado, especie en peligro Xyrauchen texanus
KEYWORDS: artificial propagation; hatcheries; genetic structure; heterozygosity; razorback sucker
ABSTRACT
For large scale artificial propagation to be an effective part of the management plans for Xyrauchen texanus,
hatchery progeny should reflect the genetic structure of the natural population. Stock sources for hatchery
propagation at the Dexter National Fish Hatchery were obtained from Lake Mohave in 1981 and 1982. Using 17
allozyme loci which had previously been found to be polymorphic in razorbacks, I examined variation in two
different year classes, and compared this variation to that found in the Lake Mohave population. The natural
population had an average level of heterozygosity of 0.099. The two year classes, produced in 1987 and 1990, had
average heterozygosities of 0.125 and 0.080 respectively, which were not significantly different from Lake Mohave.
The 1990 year class had a greatly reduced survivorship compared to previous years, and has been found to have
a significantly lower mtDNA haplotype diversity. The use of different molecular data sets in the planning and
monitoring of captive breeding programs will be discussed.
CLAVES: propagaci6n artificial; criaderos; estructura gendtica; heterocigocidad
RESUMEN
Para que la propagaci6n artificial a gran escala sea una parte efectiva de los planes de manejo para Xyrauchen
texanus, la progenie del criadero debe reflejar la estructura gendtica de la poblaci6n natural. Las fuentes del stock
para la propagaci6n en criadero en Dexter National Fish Hatchery se obtuvieron del Lago Mohave en 1981 y 1982.
Usando 17 loci de alozimas las cuales se habfa encontrado previamente que son polim6rficas para el matalote,
examin6 la variaci6n en dos clases de talla diferentes, y compar6 esta variaci6n con la encontrada en la poblaci6n
del Lago Mohave. La poblaci6n natural tiene un nivel de heterocigocidad promedio de 0.099. Las dos clases
anuales, producidas en 1987 y 1990, tienen heterocigocidades promedio de 0.125 y 0.080 respectivamente, las
cuales no fueron significativamente diferentes de las del Lago Mohave. La clase del aho 1990 tiene una
sobrevivencia fuertemente reducida comparada con los afios anteriores y se ha encontrado que tiene
significativamente menos diversidad de haplotipo de ADNmt. El use de diferentes juegos de datos moleculares en
la planeaci6n y monitoreo de programas de reproducci6n en cautiverio seran discutidos.
Ht UBBS STUDENT PAPER COMPETITORI
GORMAN,OWEN T.; TIERSCH,T.R.; WILLIAMSON,J.H.; CARMICHAEL,GJ.• (oTG - U.S. Fish and Wildlife Service, P.O.
Box 338, Flagstaff, AZ 86002-0338; TRT - Forestry, Wildlife, and Fisheries, Louisiana State University, Baton Rouge, LA 70803; JHW - U.S. Fish and Wildlife Service, Dexter NFH, P.O.
Box
219. Dexter, NM 88230; GJC - U.S. Fish and Wildlife Service, Mora NFH, P.O. Box 689, Mora, NM 87732)
Germplasm storage to preserve genetic resources in the razorback sucker and bonytail chub
Almacenaje de germoplasma para preservar recursos geneticos del matalote jorobado y el charal elegante
KEYWORDS: Cyprinidae; bonytail chub; Colorado squawfish; humpback chub; Catostomidae; razorback sucker; genetic management;
genetic resources; cryopreservation; germplasm
ABSTRACT
Germplasm storage and gene banking have proven to be valuable tools in agricultural applications and hold
great potential for applications in aquaculture. We initiated studies to expand this tool to fishes that are in danger
of extinction: bonytail chub (Gila elegans) and razorback sucker (Xyrauchen texanus). Methodologies for
germplasm preservation and storage for razorback sucker and bonytail chub were developed and tested. We
developed field techniques to collect, evaluate, refrigerate, and cryopreserve sperm from these endangered species.
Cryopreserved sperm was thawed and used to fertilize eggs. Razorback sucker and bonytail chub were successfully
produced using cryopreserved sperm.
Future plans include fine tuning developed methodologies and applying the technology to Colorado squawfish
(Ptychocheilus lucius) and humpback chub (Gila cypha). Application of these new methodologies offers great
potential for genetic management and recovery of endangered fishes.
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