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PROCEEDINGS OF THE DESERT FISHES COUNCIL, 1994 SYMPOSIUM VOLUME XXVI - published 1995 <br />FLAGSTAFF FISHERY RESOURCE OFFICE - The Flagstaff office conducted two spring and two summer <br />sampling trips into the Little Colorado River to assess/monitor stream habitat and habitat use by humpback chub <br />and other native fishes. Humpback chub appeared to commence spawning in mid-winter; appearance of a broad <br />range of YOY fishes suggested a protracted spawning season. Surveys were also conducted in Grand Canyon <br />tributaries (Pavia, Bright Angel, Shinumo; Tapeats, Deer, Kanab, Havasu) to assess/monitor stream habitat and <br />habitat use by native fishes. <br />A pilot study of cryopreservation of razorback sucker sperm was also carried out during the Lake Mohave <br />roundup, March 1994. Successfully fertilized eggs in the field with cryopreserved milt. Conducted pilot study on <br />cryopreservation of bonytail chub sperm at Dexter NFH in May; eggs were successfully fertilized with <br />cryopreserved sperm. We are preparing to conduct a pilot study on humpback chub in 1995. <br />Finally, a draft final report on GCES contracted studies was completed on June 30. A final version is expected <br />by 1 October.' <br />PARKER FISHERY RESOURCE OFFICE - Razorback suckers - To date, a total of 90 razorback suckers have <br />been released into Colorado River waters from isolated growout facilities. This includes 86 from the high levee <br />pond growout facility at Cibola NWR and 4 from Pittsburgh Point Cove on Lake Havasu. During a two week period <br />in March 230 razorback suckers were taken in the annual research on Lake Mohave by Service personnel. 24% <br />were recaptures. <br />During March-April an estimated 110,000 razorback sucker eggs were fertilized and delivered to Willow <br />Beach National Fish Hatchery. Currently, it is estimated that 10,000 fish ranging from one to 12 inches are being <br />held at the hatchery. These fish were collected from several different areas on Lake Mohave at different times of <br />the month. One to one matings were performed and a standard quanity of eggs were taken (25 in]) each time. These <br />fish were produced to determine if retrofitting of this hatchery to a warm water facility would make it feasible to <br />produce native fish. <br />Additionally, during February and March personnel from the Service participated in harvesting wild razorback <br />sucker larvae from Lake Mohave with other members of the native fish group. In conjunction with this 1200 larvae <br />were transferred to the Parker FRO and held and reared to replenish the broodstock at Dexter NFH. Two hundred <br />two-four inch fish were delivered to Dexter in October 1994. <br />During 1994 no bonytail chubs were produced from the Office Cove as it was breached shortly after <br />establishment allowing fish to escape into the lake and non-indigenous fish to enter the cove. When it was poisoned, <br />no native fish were taken. However, a total of 58 adult bonytail chub have been removed from the Hassayampa <br />River Preserve (The Nature Conservancy) and released into Lake Havasu during 1994. Six thousand 1-2 inch fish <br />were produced from 44,000 fry provided to the Parker FRO in April of 1994 and introduced into two facilities on <br />Lake Havasu. <br />During 1994, as part of the efforts of the Lake Havasu Native Fish Project in partnership with Bureau of Land <br />Management and other agencies, three additional barriered habitats were developed on Lake Havasu for native fish. <br />Additionally, a isolated habitat was developed adjacent to Lake Havasu. Two facilities in operation on Lake Havasu <br />in 1993 were also re- poisoned to prepare them for receiving bonytail chubs in October. <br />One isolated facility has also been developed on Imperial NWR. In addition to this four ponds on the La Paz <br />County golf course have been developed for native fish. <br />The Pinetop Fish Health Office examined fishes for disease from No Entry Cove and from the Hassayampa <br />River Preserve. Nothing of significance was found. However, upon examination of two bonytail chub which died <br />subsequent to the initial examination, the Asian tapeworm was found. <br />In summary, twelve native fish isolated habitats are in place along the lower Colorado River, below Davis <br />Dam. These include the following sites on or adjacent to Lake Havasu: Pittsburgh Point Cove No Entry Cove Office <br />Cove - Bill Williams River NWR Twin Cove South - Havasu NWR Twin Cove North - Havasu NWR Bulkhead <br />Cove. In addition: <br />Four ponds on the La Paz County golf course High Levee Pond - Cibola NWR Hidden Lake - Imperial NWR. <br />Currently all are stocked with bonytail chub produced by Dexter National Fish Hatchery and Technology Center. <br />The total acreage is estimated at 25 acres. <br />Besides these activities, Parker FRO has produced the Little Colorado Spinedace Recovery Plan which was <br />published in the Federal Register on 23 September 1994. The Lower Basin Management Plan for Indigenous Big <br />River Fishes was also produced and is currently under review. <br />11