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ABSTRACTS OF PRESENTATIONS IN ORDER PRESENTED
<br />interno sobre cuales agencias estan interesadas sobre el progreso de los reportes enviados al congreso, y que se puede
<br />esperar para el inicio o mediados de 1993 sobre este tema.
<br />SI IEN, Y.*; EVANS, R. P.; WrLLIAMs, R. N.; SMOZAWA, D. K. (YS, RPE, DKS - Department of Zoology, Brigham
<br />Young University, Provo, UT RNW - Department of Biology, Boise State University, Boise, ID)
<br />Restriction Fragments Length Polymorphism (RFLP) of cutthroat trout Oncorhynchus clarki
<br />mitochondria) DNA fragments amplified by Polymerase Chain Reaction (PCR) /
<br />Polimorfismo en la longitud de fragmentos de restriccion (RFLP) de la trucha garganta
<br />cortada Oncorhynchus clarki a partir de fragmentos de ADN mitocondrial amplificados por
<br />reaccion en cadena de la polimerasa (PCR)
<br />KEYWORDS: Polymerase Chain Reaction; RFLP; Oncorhynchus clarki
<br />ABSTRACT
<br />Certain regions of the mitochondria) genome have been a valuable source of information concerning genetic
<br />diversity in fish. In this study, the mitochondrial DNA (mtDNA) ORF1, ORF2, ORF5/6 and Cytochrome B gene regions
<br />of different cutthroat trout subspecies were amplified by polymerase chain reaction (PCR). PCR amplified mtDNA
<br />fragments were cleaved with 10 restriction endonucleases (AluI, Cf6I, HaeIII, HhaI, Hinfl, HpaII, MboI, MspI, Rsal,
<br />and Sau3AI). Resultant restriction fragments were separated by agarose gel electrophoresis and detected by ethidium
<br />bromide staining. Forty unique restriction fragment length polymorphisms were detected which allow unique separation
<br />of all cutthroat trout subspecies and some populations. These data were used to infer phylogenetic relationships of the
<br />cutthroat trout subspecies. The combined use of PCR and RFLP techniques provides a rapid and nonlethal technique for
<br />the identification of native cutthroat trout subspecies.
<br />RESUMEN
<br />Ciertas regiones del genoma mitocondrial han sido una fuente valiosa de information en el estudio de la diversidad
<br />genetica en peces. En este estudio, se amplifican por medio de la reaccion en cadena de la polimerasa (PCR), ]as regiones
<br />genicas ORF1, ORF2, ORF5/6, y citocromo B del ADN mitocondrial (ADN mt) de diferentes subespecies de trucha
<br />garganta cortada. Los fragmentos de ADN mt amplifrcados por PCR f teron cortados con 10 endonucleasas de restriccion
<br />(Alul, CfoI, HaeIII, HhaI, Hinfl, HpaII, MboI, MspI, RsaI, y Sau3AI). Los fragmentos de restriccion resultantes fueron
<br />separados por electroforesis en gel de agarosa y detectados por medio de la tincidn con bromuro de etidio. Cuarenta
<br />polimorfismos de longitud de los fragmentos de restriccion fueron detectados, los cuales permitieron la separaci6n de
<br />todas ]as subespecies de trucha garganta cortada, como tambien de algunas poblaciones. Estos datos fueron utilizados
<br />para inferir ]as relaciones filogeneticas de las subespecies de trucha garganta cortada. El use combinado de las tecnicas
<br />de PCR y RFLP, proveen un procedimiento rapido y no letal para la identificacidn de subespecies nativas de trucha
<br />garganta cortada.
<br />GORMAN, OWEN T.*; LEON, STUART C.; MAUGHAN, O. EUGENE (OTG - U.S. Fish and Wildlife Service, P.O. Box
<br />338, Flagstaff, AZ 86002-0338; SCL - U.S. Fish and Wildlife Service, P.O. Box 39, Pinetop, AZ 85935; OEM - Arizona Cooperative Fish and Wildlife
<br />Research Unit, University of Arizona, Tucson, AZ 85721)
<br />Habitat use by humpback chub, Gila cypha, in the Little Colorado River and other
<br />tributaries of the Colorado River / Uso de habitat del charalito jorobado, Gila cypha, en
<br />el Pequeno Rio Colorado y otros tributarios del Rio Colorado
<br />KEYWORDS: Cyprinidae; Gila cypha; ecology; streams; habitat; endangered species; Grand Canyon; Arizona
<br />ABSTRACT
<br />The objective of USFWS research is to determine habitat use by the endangered humpback chub and other native
<br />fishes in the Little Colorado River (LCR) and other tributaries of the Colorado River in the Grand Canyon, evaluate the
<br />potential for establishing a second spawning aggregation of humpback chub, and from the perspective of habitat
<br />requirements, evaluate the impact of Glen Canyon dam on native fishes. Research commenced in the LCR in July 1991
<br />and in January 1992 for other tributaries. Stream habitat in the lower 21 km of the LCR and the lower 8 km of other
<br />tributaries has been mapped and sampled. Habitat use patterns were evaluated from seining, electrofishing, and
<br />hoop netting. In the LCR, humpback chub were the predominant species and maintained a resident population. Preliminary
<br />analyses indicate that adult chubs are highly habitat specific and that juvenile chub show a very different pattern of habitat
<br />use.
<br />RESUMEN
<br />El objetivo de la investigation del USFWS es determinar el use del habitat del charalito jorobado en peligro de
<br />extincidn y otros peces nativos del Pequeno Rfo Colorado (PRC) y otros tributarios del Rio Colorado en el Gran Canon,
<br />evaluar el potential para el establecimiento de una segunda agregaci6n de desove del charalito jorobado, y de la
<br />perspectiva de requerimiento de habitat, evaluar el impacto de la presa del Canon Glen en los peces nativos. Las
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