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ABSTRACTS OF PRESENTATIONS IN ORDER PRESENTED <br />En este estudio investigamos la variabilidad (43 loci) en C. formosa de 5 cuencas y C. bocagrande en Ojo Solo. <br />Usando Cyprinella lepida y Cyprinella lutrensis como grupos externos de comparaci6n, hip6tesis filogeneticas (distancia <br />de Wagner, PAUP, FREQPARS) fueron usadas para evaluar modas similares de especiaci6n y tasas de divergencia que <br />operan dentro de este grupo de especies. <br />LANG, B. K.*; PLATANIA, S. P. (UNM Ichthyofaunal Studies Program, DepaRment of Biology, University of New Mexico, Albuquerque, NM) <br />Current distribution and status of the Rio Grande silvery minnow, Hybognathus amarus / <br />Distribucian actual y situaci6n del charalito plateado del Rio Grande, Hybognathus amarus <br />KEYWORDS: Hybognathus amarus; Rio Grande; New Mexico <br />ABSTRACT <br />The Rio Grande silvery minnow, Hybognathus amarus, has experienced a 95 % reduction in its historic range and <br />currently is known only from the Middle Rio Grande in New Mexico. Demands by water users, fluctuations in regional <br />precipitation patterns, and the decline in native fish populations stimulated the need for systematic survey to determine <br />the conservation status of the middle Rio Grande ichthyofauna with specific focus on H. amarus. The first phase of a <br />five-year cooperative study was initiated in 1992 and designed to document the present distribution and status of H. <br />amarus. The study area consisted of three distinct zones, demarcated by drainage diversion structures, along a 186 km <br />reach of the middle Rio Grande from Cochiti Reservoir downstream to Elephant Butte Reservoir. Hybognathus amarus <br />was taken in 46 of 81 (57%) mainstem Rio Grande collections and one irrigation canal return site. Over 82% (38 of 46) <br />of these occurrences were in the southern two regions (downstream of Isleta Diversion Dam) of the study area. While <br />eight sites upstream of Isleta Diversion Dam yielded H. amarus, none were taken in the 25 km reach between Angostura <br />Diversion Dam and Cochiti Reservoir. <br />RESUMEN <br />El charalito plateado del Rio Grande, Hybognathus amarus, ha experimentado una reducci6n del 95 % de su area <br />hist6rica de distribuci6n y actualmente se conoce solo de ]a regi6n central del Rio Grande en Nuevo M6xico. La demanda <br />de agua por usuarios, ]as fluctuaciones en los patrons regionales de precipitaci6n, el decline de las poblaciones nativas <br />estimularon la necesidad de estudios sistematicos para determinar el estado de conservaci6n de la ictiofauna de la regi6n <br />central del Rio Grande enfocado especificamente sobre H. amarus. La primera fase de un estudio cooperativo de cinco <br />anos fue iniciado en 1992 y fue disehado para documentar la distribuci6n actual y situaci6n de H. amarus. Este estudio <br />consisti6 de tres zonas distintas, demarcadas por ]a estructura de diversion de la cuenca, a to largo de un segmento de <br />186 km de la regi6n central del R. Grande desde la presa Cochiti rfo abajo hasta la Presa Elephant Butte. Hybognathus <br />amarus fue capturado en 46 de 81 (57%) colectas en el cauce principal del R. Grande y en un sitio dentro de un canal <br />de irrigacion. Mas del 82% (38 de 46) estas ocurrencia fueron en las dos regiones sur (rfo abajo de la Presa de <br />derivaci6n Isleta) del area de estudio. Mientras que en ocho sitios rfo arriba de la Presa de Derivaci6n Isleta se encontr6 <br />H. amarus, ninguno fue capturado en el segmento de 25 km entre ]as Presas de Derivaci6n Angostura y Cochiti. <br />NAKAGAWA, P. A.; SOLTz, D. L.*; SANDERS, B. M. (PAN and BSM - Molecular Ecology Institute and Department of Biology, <br />CSULB, Long Beach, CA; DLS - Department of Biology, CSULB, Long Beach, CA) <br />The Role of Stress Proteins in the Physiological Adaptation of the Amargosa Pupfish, <br />Cyprinodon nevadensis / El papel de las proteinas "stress" en la adaptaci6n fisiol6gica del <br />cachorrito Amargosa Cyprinodon nevadensis <br />KEYWORDS: chaperonin; Cyprinodon; hsp70; heat-shock proteins; stress-70; stress proteins; thermotolerance <br />ABSTRACT <br />The cellular stress response or heat-shock response, is involved in protecting organisms from damage as a result <br />of exposure to a wide variety of environmental stressors. The response entails the rapid synthesis of a suite of proteins <br />referred to as stress proteins. These proteins are ubiquitous and highly conserved. Their synthesis is closely correlated <br />with acquired tolerance in which a conditioning heat shock confers the ability to survive a subsequent, more severe heat <br />shock that otherwise would be lethal to the organism. Two of these proteins, stress-70 and chaperonin, are molecular <br />chaperones which under normal conditions help maintain protein homeostasis. They also repair and protect other cellular <br />proteins from temperature induced damage. <br />Surprisingly, little is known about the role of stress proteins in organismic adaptation under natural environmental <br />conditions. If stress proteins are involved in this adaptation then we reasoned that an extensive stress protein response <br />would occur in species which have successfully colonized more unpredictable and extreme environments. Further, <br />studying differences in the stress response, perhaps differences in induction of stress protein synthesis or the appearance <br />of unique proteins, between subspecies with differential tolerances to environmental factors might allow the identification <br />of successful adaptive strategies. <br />To this end we have conducted an intraspecific study of the stress response in two subspecies of Amargosa pupfisb <br />Cyprinodon nevadensis which occupy different habitats. One subspecies, Cyprinodon nevadensis amargosae, lives in a <br />18