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Table 3. Loci fixed among the RBS, with the four FMS being polymorphic and sharing the <br />single RBS allele. <br />Loci All 1P rrPr Pond o O ray CO Green River 1T FMS <br />ACP-1 * 1 1.000 1.000 1.000 0 <br />500 <br /> 2 0.000 0.000 0.000 . <br />0.500 <br />K-B2* 1 0.000 0.000 0.000 0 <br />125 <br /> 2 1.000 1.000 1.000 . <br />0.875 <br />PI-A2* 1 1.000 1.000 1.000 0.875 <br /> 2 0.000 0.000 0.000 0.125 <br />IDHP-B2* 1 1.000 1.000 1.000 0 <br />875 <br /> 2 0.000 0.000 0.000 . <br />0.125 <br />PGDH-1 * 1 1.000 1.000 1.000 0 <br />750 <br /> 2 0.000 0.000 0.000 . <br />0.250 <br />PROT-1 * 1 0.000 0.000 0.000 0.500 <br /> 2 1.000 1.000 1.000 0.500 <br />Table 4. The locus for which both RBS and FMS were polymorphic. <br />Locus All .lP Etter Pond O O rraY CO Green River. JUT FMS <br />LDH-B 1 * 1 0.607 0.583 0.724 0.000 <br />2 0.000 0.000 0.000 0.250 <br />3 0.393 0.417 0.276 0.750 <br />The bottom line for the Etter Pond objective is that the results for the nine fixed loci <br />indicate conclusively that the Etter Pond fish are in no way recent hybrids; the results at <br />K-A*, MDH-A 1 *, IDHP-A* and (perhaps ADA*) presented in Dr. Buth's preliminary report <br />likely represent only shared alleles between the two species. This sharing may or may not be the <br />result of some type of non-recent hybridization events. <br />In analyzing YOY from the families produced from the Etter Pond fish, we have had <br />some difficulty aligning our observed genotypes with those reported for the numbered parents <br />(Buth's preliminary report). <br />4