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<br />3 <br />(including those not receiving food) were siphoned clean of <br />accumulated food and organic material and inspected for dead fish <br />prior to each feeding. Dead larvae recovered were preserved in <br />5% buffered formalin. Not all dead fish were rer_.ovm-red, in part <br />because of their small size and raaid rate of decomposition; some <br />must have floated to the surface of far, become lodged beneath <br />the screen and not detected; and a few were inadvertantly killed <br />by siphoning. Fish not recovered and thus unaccounted for were <br />considered mortalities. All recovered and observed mortalities <br />were recorded by date, and if possible, later measured. Larvae <br />surviving to ends of experiments were preserved and measured. <br />Experiment 1, Starvation. This experiment was designed to <br />determine when larval mortalities occurred in total absence of <br />food. Six bars were stocked with 10 individuals each. <br />Experiment 2, Time to Irreversible Starvation. Time to <br />irreversible starvation, marked by mortalities >50%, was <br />identified from larvae stocked in aquaria and started on nauplii <br />(300 per liter concentration) at 4-day intervals beginning day 5 <br />post-hatching and ending on day 29. The experiment was <br />replicated 3 times. <br />Experiment 3, Effect of Ration Size. Mortality was investigated <br />for larvae fed Artemia nauplii concentrations of 5, 10, 50, 100, <br />500 and 1,000 per liter, with 3 replicates each. To estimate <br />minimum prey density, mortalities not accounted for (see above) <br />were distributed over dates when mortalities were known to occur