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We are maintaining the five adult bonytail, 500 two-year-old brood, and 1800 <br />one and two-year-old fish scheduled for stocking in Arizona waters. <br />1 Other Catostomids - We continue to maintain one Yaqui sucker with the RBS brood <br />while we await clearance to bring in more fish from Mexico. <br />Other C rinids - We continue to maintain two populations of Chihuahua chub <br />Mimbres River, NM and Rio Pierdras Verde, Chihuahua, Mexico). The Mexican <br />government has requested that we rear fish for them in 1994. Groundwork <br />preparatory to restocking fish in the Mimbres seems to be progressing and a <br />1984 stocking appears to be possible. Experimentation with hormone-induced <br />spawning of Chihuahua chub was successful in 1983. No data was collected on <br />the brief experiment but it was determined that this species can he spawned <br />for mass production if required. Preserved specimens were provided to nr. Jim <br />Sublette, ENMU and Dr. Robert R. Miller, U of M Museum of Zooloqy. We also <br />prepared several specimens for skeletonization for Or. Miller. <br />We continued to experiment with natural spawninq of woundfin in ponds. We <br />only carried over 15 fish from 1982 so an additional nh0 fish were collected <br />from the Virgin River to bolster our stock. The 475 fish were stocked in a <br />prepared spawning pond on larch 24. On June 29 we transferred 375 adults and <br />870 fry to a new prepared spawning pond; we had hoped the adult fish would <br />spawn again but they didn't. On August 31 we moved 346 adults and 291 iuveniles <br />to a new pond. On November 2 we moved a total of 200 fish to a holding pond <br />for the winter. Unless things change from previous years, we can anticipate <br />or greater loss of the remaining fish during the winter. Although woundfin <br />are quite small, we will probably have to experiment with induced spawning in <br />/ order to produce adequate numbers of fish for stocking. Preserved spcimens were <br />provided to Dr. Sublette. <br />We maintained populations of the Yaqui chub and beautiful shiner; both have <br />maintained adequate numbers through natural reproduction. Specimens were pro- <br />vided for Dr. Sublette's collection and to Dr. W. 0. Minckley, ASU for con- <br />firmation of species genetic integrity. <br />Poeciliids - We maintained populations of Big Bend gambusia and Gila topminnow. <br />Both species have done well, producing large populations. The Yaqui topminnow <br />did not overwinter outside and per Regional SE directives, the stock held <br />inside was preserved and the species removed from station inventory. Good <br />numbers of fish in waters on the San Bernardino NWR allowed this action. One <br />hundred live fish of all three species were shipped to Dr. Jay Stauffer, <br />Appalachian Environmental Research Lab, Frostberq, Maryland and 200 live Gila <br />topminnows to Dr. John Burns, George Washington University, Washington, n.C. <br />Big Bend gambusia specimens were collected by Dr. Clark Hubbs for a special <br />study and additional specimens sent to him later to confirm genetic integrity. <br />Cyprinodontids - We maintained populations of the Leon Springs, Comanche Springs <br />and desert pupfish. Both the Leon Springs and Comanche Springs pupfish continue <br />to produce and maintain adequate numbers of fish. One hundred live fish of both <br />these species were shipped to Dr. Stauffer at AERL. A few preserved Leon Springs <br />and Comanche Springs pupfish were shipped to Dr. Hubbs for confirmation of species <br />integrity. Troy and Roger went into Sonora, Mexico with Jerry Burton and Mexican <br />officials to pick up the desert pupfish. They returned to Dexter on April 29 with <br />280 desert pupfish of various sizes. This species has done extremelv well with <br />thousands of young fish being produced durinq the summer.