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Last modified
7/14/2009 5:02:29 PM
Creation date
5/20/2009 9:27:52 AM
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UCREFRP
UCREFRP Catalog Number
7132
Author
Jensen, B. L.
Title
Culture Techniques for Selected Colorado River Imperiled Fishes.
USFW Year
1983.
USFW - Doc Type
December 6-8, 1983.
Copyright Material
NO
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injection. Females receive HCG injections every 24 hours until <br />all eggs.have been ovulated. At the recommended dosage, they <br />usually give a "show" of eggs at 36 hours and good spawns at 48, <br />60 and 72 hours; some females spawn out at 60 hours. Male razor- <br />backs are injected with HCG at 300 IU per pound of body weight <br />daily for two to three days. If milt production decreases, HCG <br />injections are reinitiated to maintain production of male gametes. <br />Utilizing the wet method, eggs from ripe females are hand- <br />stripped into pans, milt added from two or more males and the eggs <br />stirred gently with a feather. Razorback sucker eggs are very <br />adhesive so the eggs are "clayed" with slurried bentonite following <br />fertilization. The clayed eggs are poured into floating egg baskets, <br />gently washed to remove the bentonite and allowed to water harden for <br />30 minutes. Following water hardening, they are enumerated gravi- <br />metrically and placed in Heath incubator trays. Jar hatching experi- <br />ments revealed that razorback sucker eggs are too fragile to withstand <br />the rolling involved in this hatching technique (Inslee, in press). <br />Marsh and Pisano (in press) conducted studies at Dexter to determine <br />the influence of temperature on egg development and hatching success <br />of native Colorado River fishes. They found that optimum incubation <br />temperatures for eggs of the three species reported here was near <br />700F. Consequently, our well water is heated to 700F for egg incu- <br />bation and fry development to swimup. Water flow through the Heaths. <br />is regulated at three gpm. At 700F razorback sucker eggs begin <br />hatching at about 96 hours and continue through 144 hours; peak <br />hatching occurs on the fifth day at about 120 hours. Fry are held <br />in tanks until swimup, then stocked in rearing ponds. Stocking rates <br />for razorback sucker fry have not been finalized; we have experimented <br />with rates ranging from 80,000 to 200,000 per surface acre. <br />In 1983 we spawned a total of 55 wild razorback sucker females <br />46 of them (83.6%) successfully. The 46 fish produced 5,728,025 <br />eggs for an average fecundity of 124,522. Egg viability at 72 hours
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