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elevation sites had an_ average of 5 species <br />of fish, which were designated a trout <br />stream assemblage in the cluster analysis of <br />fish presence/absence data. The sites at the <br />upper end of the distribution were generally <br />below reservoirs or were large river sites <br />(e.g., lower elevation, mainstream Rio <br />Grande sites). The histogram suggests that <br />in development of an IBI, separate indices <br />may --need to - be developed -for "trout <br />streams" and large river/reservoir tailwater <br />sites. <br />To explore further the relationship between <br />number of fish species and elevation, data <br />from the upper Rio Grande drainage were <br />compared with data from a study of the <br />fishes in the Black River, Eddy County, <br />New Mexico (Cowley, 1979; Cowley and <br />Sublette, 1987). The linear regression of <br />number of species onto elevation for sites <br />on the Black River are plotted together with <br />the upper Rio Grande data in Figure 27. <br />The individual regressions for upper Rio <br />Grande data and Black River data differed <br />significantly in slope and in intercept. This <br />indicated that substantially different <br />relationships between fish species number <br />and elevation can be expected in different <br />regions of the state. As a result, it will <br />probably be necessary to develop different <br />IBIs for different ecological regions of New <br />Mexico. The data also suggested that <br />elevation alone will be insufficient to <br />account for fish species diversity across a <br />large and physiographically heterogeneous <br />region such as New Mexico. <br />Finally, the potential utility of the distance <br />(km) from headwaters was investigated. <br />Here, number of species per site for Black <br />River was regressed onto distance of the site <br />from the headwaters (Figure 28). The <br />improved fit of the data (W=0.82), relative <br />to the comparable regression against <br />elevation, suggested that distance from <br />headwaters may have wider applicability <br />across different regions than elevation. <br />Discussion <br />Upper No Grande Aquatic <br />Ecoregions <br />The two aquatic ecoregions described in this <br />report have a boundary somewhere in the <br />vicinity of 7400 feet (Figure 6). This <br />elevational division is very close to the <br />ecoregion boundary in the upper Rio <br />Grande drainage that was described by <br />Omernik (1986, 1987; Figure 7). Omernik <br />described a Southern Rockies Ecoregion <br />that is consistent with Aquatic Ecoregion 1 <br />defined through multivariate ordination and <br />clustering. Omernik's - Arizona/New <br />Mexico Plateau Ecoregion appears to be <br />reasonably consistent with Aquatic <br />Ecoregion 2 in the upper Rio Grande. The <br />aquatic ecoregions are less similar to <br />ecoregions defined by the Forest Service <br />(Bailey, 1994a,b; Bailey et al., 1994). <br />It is interesting to compare the results <br />obtained for the upper Rio Grande drainage <br />of New Mexico with studies conducted <br />elsewhere. The first multivariate <br />environmental factor obtained for upper Rio <br />Grande sites, elevation and variables that <br />vary inversely with elevation, is very <br />similar to the first factor obtained for <br />Arkansas streams (Matthews et al., 1992). <br />In a study of Swedish streams, the most <br />important environmental variables were <br />drainage area, elevation, alkalinity, color, <br />and phosphate. The most important <br />multivariate environmental factors for the <br />31