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Preliminary IBI Statistics <br />As part of the analyses for this report, the <br />potential utility of several summary <br />statistics for development of an IBI was <br />investigated. These statistics include: (1) <br />number of unique chironomid species, (2) <br />frequency distributions for number of <br />chironomid species and number of fish <br />species, (3) additional investigations into <br />number of fish species versus elevation, and <br />(4) the potential utility of an additional <br />environmental variable, distance from <br />headwaters. <br />The number of unique chironomid species <br />per site was regressed onto the total mmaber <br />of chironomid species per site (Figure 23). <br />The significant linear regression (R2=0.70) <br />indicated that about one-fourth of the <br />chironomid species at a site can be expected <br />to be found nowhere else. This level of <br />biotic site-specificity is probably unequaled <br />among any other group of animals. It <br />underscores the potential utility of <br />chironomids as indicators of habitat quality. <br />The strong linear relationship suggests that <br />the total number of chironomid species, the <br />number of unique chironomid species, or <br />both, could provide useful summary <br />information for inclusion in an IBI. It also <br />suggests that for a given number of species, <br />sites that lie above the regression line may <br />prove to have higher integrity than sites that <br />fall below the line. In anticipation of <br />inclusion of chironomid species in an IBI, a <br />manuscript handbook of keys and <br />illustrations of the pupae of New Mexican <br />chironomids is being prepared (Appendix <br />4). <br />A frequency distribution for number of <br />chironomid species was produced where <br />numbers of species were grouped into <br />ranges of 10 (Figure 24). Assuming that <br />the number of chironomid species is <br />correlated positively with environmental <br />integrity, the histogram indicates that <br />integrity at most of the upper Rio Grande <br />sites is moderately to highly degraded, and <br />that only a few sites have a high diversity of <br />chironomid species. The disjunct nature of <br />the distribution suggests that if the number <br />of chironomids at a site were to be included <br />in an IBI, a threshold indicator function <br />might be appropriate. For example, given <br />the break in the distribution above .30 <br />species at a site, one might give a site a <br />higher integrity score if it has 30 or more <br />chironomid species. The distribution of <br />chironomid subfamilies with respect to <br />elevation (Box 3) suggests that a metric <br />based on subfamily might have utility in an <br />IBI. <br />The frequency distribution for benthic <br />macroinvertebrate taxa (Figure 25) suggests <br />bimodality, i.e., one grouping of sites with <br />10 to 20 benthic macroinvertebrate taxa and <br />a second grouping of sites with more than <br />20 benthic macroinvertebrate taxa. The <br />break in the distribution suggests that sites <br />having more than 20 benthic <br />macroinvertebrate taxa may have better <br />integrity than sites having fewer taxa. <br />Figure 26 provides a histogram for the <br />frequency distribution of number of fish <br />species per site. Two headwater stream <br />sites in the upper Rio Grande drainage had <br />only one fish species, a condition consistent <br />with the generally depauperate fish fauna in <br />headwater systems (e.g., Stewart and Loar, <br />1994). The remainder of the distribution <br />displayed bimodality. The modes in the <br />distribution are consistent with the <br />observation from cluster analysis of a <br />mid-elevation group of sites and a lower <br />elevation group of sites. The middle <br />3?0 <br />19