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disagreement distances <br />using Ward's method.' <br />ethod. <br />Fish Species Associations <br />between species Species-Environment RehWons <br />Two cluster analyses were conducted to <br />illuminate associations of fish species. <br />First, cluster analysis on presence/absence <br />data was made using Jaccard dissimilarity <br />distances - between species -and -Ward's <br />method. This analysis provided <br />assemblages of fish species in the upper Rio <br />Grande. <br />Jaccard's similarity coefficient (Sneath and <br />Sokal, 1973; Bridge, 1993) minimizes <br />errors when a large number of negative <br />results occur, e.g., when two species are <br />found only at a few of the sites, a large <br />number of the pairwise data are negative <br />(both absences). Inclusion of the negatives <br />gives a similarity that is too large and <br />conversely, a distance that is too small. <br />The Jaccard dissimilarity distance was <br />obtained as the converse of the Jac card <br />similarity coefficient (Bridge, 1993). The <br />Jaccard dissimilarity provided better <br />resolution than the percent disagreement <br />distance for upper Rio Grande fish species <br />because a number of fish species were <br />found at only one or a few sites. A <br />FORTRAN Program (Appendix 3) was <br />written and used to obtain the Jacquard <br />dissimilarities. <br />Cluster analysis was performed using <br />relative abundance data. In this analysis, <br />Euclidean distances and Ward's method <br />were used. This analysis, in contrast to the <br />analysis of presence/absence data, does not <br />provide a true picture of the assemblages of <br />fish species. Rather, it provides groupings <br />of abundant and less abundant species. <br />Species-environment relations were <br />examined in several ways. First, for fish <br />data, canonical correspondence analysis <br />(CCA) (ter Braak, 1985, 1986, .1987a,b; <br />Taylor et al., 1993) and detrended CCA <br />(DCCA) (ter Braak and Barendregt, 1986; <br />ter Braak and Looman, 1986; ter Braak and <br />Prentice, 1988) was ..performed .. using <br />CANOCO (ter Bmak, 1987-1992). <br />Canonical correspondence analysis is an <br />iterative combination of ordination and <br />multiple regression that attempts to relate <br />species distribution and abundance to <br />environmental variables. Detrended CCA <br />takes the results from CCA and rescales the <br />variation in such a way that the biplot can <br />provide a finer scale view of <br />species-environment relations. The end <br />result of CCA and DCCA is a biplot <br />(Gabriel, 1971, 1981) which allows <br />simultaneous viewing of axes of <br />environmental variation and ordination of <br />species with respect to those axes of <br />environmental variation. Prior to analysis <br />by CCA and DCCA, fish species relative <br />abundance data were ranked within sites by <br />assigning a value of one to the least <br />abundant species up to a value equal to the <br />number of species found at the site for the <br />most abundant species. Twenty-one of the <br />22 environmental variables were included in <br />CCA and DCCA; the categorical variable <br />representing land use designation was <br />omitted. <br />Second, the relationship between elevation <br />and the number of fish species was <br />investigated with linear regression. <br />Comparable regressions were performed for <br />elevation and the number of chironomid <br />species elevation and the number of benthic <br />macroinvertebrate taxa. Third, the <br />congruence of fish faunal regions, <br /> <br />7