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urogenital opening, T. Chart, personal communication; Chart and Lentsch 1999b). After <br />handling, Gila were placed into a 1.5% salt bath for 0.5-1 min and then released at the central <br />processing point. About 9% of the humpback chubs handled were held overnight in a soft- <br />mesh live cage to assess short-term mortality. <br />Data Analysis <br />Mean catch per effort for trammel netting (CPE, fish per net hour) was calculated for <br />humpback chub and roundtail chub for each sampling rotation within this study and for earlier <br />ISMP sampling (1988, 1991, 1994, 1997; McAda et al. 1994). Mean CPE was compared <br />among individual sampling efforts with the Kolmogorv-Smirnov two sample test after initial <br />differences were established with analysis of variance. Linear regression was used to estimate <br />trends in mean CPE over time. All statistical testing was done with SYSTAT (Wilkinson <br />1997); significance was accepted at Ps0.05. Mean CPE was not calculated for electrofishing <br />or angling because of limited effort. <br />Capture-recapture data for all humpback chubs handled were placed into a matrix <br />organized by sampling rotation. Population estimates were made for adult humpback chubs <br />(z 200 mm TL, USFWS 2002) and for all humpback chubs collected (all sizes combined). The <br />matrix identified whether a specific humpback chub was captured or not during each sampling <br />rotation. The population estimation program CAPTURE (White et al. 1982) was then used to <br />calculate annual abundance estimates for humpback chub in Black Rocks. CAPTURE allows <br />a variety of different models to be run and provides a model selection algorithm to suggest the <br />best model for the data set used. After estimates were generated for each year sampled, an <br />average was calculated for the 3 years to provide a population estimate for the study period. <br />Length-frequency distributions were calculated for each year of sampling. Humpback <br />chubs recaptured within a year were only used once in the analysis. Length-frequency <br />distributions were also calculated for earlier ISMP sampling. Sex ratio was calculated for fish <br />handled during the last rotation of 1999; chi-square analysis was used to determine if the ratio <br />was significantly different than 1:1. Annual growth was estimated by comparing total length <br />of recaptured fish that had been handled in previous years. <br />4 <br />