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in the upper basin will be assessed and monitored using appropriate <br />methods such as electrophoresis for protein analyses and restriction <br />enzyme analysis for DNA. Other criteria such as geographic distribution, <br />relative abundance, identified spawning sites, migration, and movements <br />between stocks will also be used to identify and characterize stocks. In <br />' some cases, morphology may be useful in identification and <br />characterization of stocks. <br />' All proposed stocking activities (i.e., experimental, augmentation, or <br />restoration stocking) in the upper basin will be conducted as experiments <br />that can be thoroughly evaluated to ensure that stocking will not disrupt <br />the genetic diversity of remaining wild stocks and to assess the <br />potential success of stocking. Habitat enhancement activities will be <br />implemented before or in conjunction with stocking and will also be <br />conducted as experiments to evaluate the responses of all fish species. <br />These actions are necessary to prevent hybridization or introgression <br />among and within similar species and to minimize competition and <br />predation by nonnative fishes on the endangered fishes. <br />It is especially important to limit experimental stocking of captive- <br />reared endangered fishes to evaluate the benefits of stocking and to <br />avoid potential adverse affects on genetic diversity of wild stocks <br />(Kapuscinski et al. 1993; Philipp et al. 1993; Riggs 1990). Lichatowich <br />and Watson (1993) emphasized that stocking of captive-reared endangered <br />fishes may be a useful management tool but should not be used as a <br />substitute for the protection and restoration of habitat to reestablish <br />viable wild populations. They state that management emphasis on existing <br />stable wild stocks of endangered fishes should be directed primarily on <br />protection, preservation, and enhancement of natural habitats needed by <br />various life stages to increase the numbers of fish that are needed for <br />recovery. <br />B. Declining Stocks. A systems approach (Box 3) will be used for (1) <br />inventory and scoping (i.e., assessment) of available information and <br />identification of issues or problems, (2) strategic planning for <br />establishing measurable objectives, determining possible solutions to the <br />issues or problems, reviewing opportunities for genetics management, and <br />evaluating the potential genetics risks, (3) operational planning to <br />implement necessary actions, and (4) methods for evaluation to determine <br />the degree of success or to make necessary refinements. Factors that may <br />be limiting numbers of the endangered fishes should be identified and <br />actions taken to remove or significantly reduce the factors that caused <br />population declines to achieve the overall goal of recovery. Emphasis <br />will be placed on natural propagation of wild endangered fish stocks so <br />that they can become self-sustaining. The relationship of captive <br />propagation and stocking to natural propagation is outlined in Figure 1. <br />All captive-reared fish for experimental, augmentation, or restoration <br />stocking will be propagated using procedures outlined in Section V <br />"Genetics Management of Captive Stocks" to maintain genetic diversity <br />that is similar to the wild stock used as founders of broodstocks. <br />13