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.' <br />method would have to incorporate recent data that demonstrate extensive <br />movements of the young fish from backwaters to other habitats in response <br />to differences in water temperatures, and perhaps other variables as well. <br />Such movements confound the results of simple seining studies, some of <br />which have suggested higher concentrations of young fish in the Spring <br />than in the previous Autumn. <br />FWS studies in 1980-1981 (Tyus 1991) demonstrated that young Colorado <br />squawfish could be marked and recaptured weeks later. We would evaluate <br />the use of other marking methods for fish recognition, and select a non- <br />lethal mark that could be retained for a least 6 months. The <br />effectiveness of this mark and its effects on the fish would be evaluated <br />by using hatchery reared fish. <br />Existing data and information on: the effects of environmental variables <br />on habitat selection in young Colorado squawfish, differences in <br />behavioral patterns, differences in relative abundance of various size <br />classes of young squawfish, and mark/recapture data would be used to <br />design a new plan of study that would be proposed to further evaluate data <br />gaps for 1992. The basis for this issue paper would be an evaluation of <br />standing crops of various size classes, and annual growth increments of <br />fish that have been previously aged. <br />This work can best be accomplished in the Green River because of the long- <br />term extensive database, and large standing crops of the fish. <br />VI. Task Description (FY-1992): <br />Task 1: Evaluate binary coded wire tags and half tags as non-lethal <br />marks for Colorado squawfish 40-70 mm TL by conducting a <br />laboratory mark retention/recognition study on hatchery fish. <br />Task 2: Conduct a pilot field study to determine the feasibility of <br />estimation overwinter survival by making riverwide population <br />estimates of age-0 fish in Autumn and age-1 fish in spring <br />prior to spring runoff. <br />a.) Estimate backwater population size of age-0 or age-1 <br />fish by randomly selecting backwaters and make a <br />population estimate for those backwaters and measure <br />backwater area. <br />b.) Use videography techniques to determine total backwater <br />area -available <br />c.) For each estimate, determine fraction of young Colorado <br />squawfish using backwaters by sampling shoreline, <br />eddies, and other types of river habitat as well as <br />backwater habitat. <br />Task 3: Design a long term study to relate overwinter survival and <br />winter flows <br />VII. Study Schedule (start/end by task): <br />1. Evaluate non-lethal mark <br />2. Pilot field study <br />a. Autumn estimate: <br />b. Spring estimate: <br />3. Study design: <br />4. Final report: <br />1 Aug 91 - 15 Apr. 92 <br />30 Oct. 91 <br />15 Apr. 92 <br />1 Oct. 92 <br />15 Mar. 93 <br />VIII. FY-92 Work <br />- Description of work: <br />- Deliverables: <br />Evaluate non-lethal <br />study, study design. <br />annual report <br />C10 <br />mark, pilot field