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<br />of razorback sucker, bonYtail, humpback chub, and Colorado squawfish are <br />illustrated in Figures 5, 6, 7, and 8, respectively. <br />' B. Space and Water Requirements for Indoor Culture of Fish During the First <br />Growing Season. Culture of the endangered Colorado River fishes during <br />the first growing season can be accomplished by rearing the fish indoors <br />for the entire period (See Sections "1-4" below), a combination of indoor <br />and outdoor culture (See Section "5" below), cage culture in outdoor ponds <br />(See Section "6" below), or off-channel growout ponds (See Section "7" <br />below). However, it should be noted that there are advantages and <br />disadvantages to intensive and extensive rearing of fish. Either method <br />may be used in the culture of the endangered Colorado River fishes but <br />should be thoroughly evaluated to determine the best method for culturing <br />these species. <br />1. Indoor Feeding Requirements and Growth of Fish. Many factors enter <br />into the hatchery requirements (i.e., space and water) in the indoor <br />culture of fish. Water quality and quantity are key factors for <br />' selection of a fish propagation site. Water quantity places a limit <br />on the total production of fish. Production can be increased if the <br />water quantity allows greater water exchange to remove metabolic <br />wastes such as unionized ammonia that is highly toxic at warm water <br />temperatures and at high pH values. Water quality is the most <br />critical factor in propagation of fish since water temperature, <br />dissolved gases, pH, suspended solids, acidity, alkalinity, dissolved <br />minerals, and contaminants affect the well-being of fish. The <br />experience of the hatchery manager and assistant manager is vital in <br />operation and management of hatchery facilities. Numerous references <br />can be obtained from the published literature that explain the <br />importance of water quantity and quality, culture techniques, loading <br />densities, feeding rates, etc. to effective operation and management <br />' of fish hatcheries (E.g., Allen and Kinney 1981; Bell 1986; Colt and <br />White 1991; Johnson and Jensen 1991; Hamman 1981x, 1981b, 1982, 1985, <br />1986, 1987, 1989; Haskell 1959; Piper et al. 1982; Parker and Davis <br />' 1981; Stickney 1993; Toney 1974, 1992; Westers 1985, 1989). Numerous <br />other references are available on fish health, nutrition, anesthetics <br />and drugs, stress related to handling and transporting fish, intensive <br />culture, and extensive culture. <br />It would not be feasible to attempt to describe these factors in <br />detail in this plan. However, a brief explanation provides insight <br />' into how these factors are used to determine loading rates of fish <br />based on water flow and fish growth. The following recommendations on <br />density of fish, dissolved gases, flow, food, and growth are provided <br />for endangered Colorado River fishes reared in indoor troughs through <br />' the first growing season: <br />Dissolved gases. Nitrogen and oxygen are the two most abundant gases <br />' that dissolve in water. Although the atmosphere contains about four <br />times more nitrogen than oxygen in volume, freshwater normally <br />contains about twice as much nitrogen as oxygen when in equilibrium <br />' with the atmosphere because of the difference in solubility between <br />the two gases. <br />45