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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />T bl 10 <br /> <br />B t h' <br /> <br />f <br /> <br />b k k <br /> <br />d' 1997 <br /> <br />a e . a c 102 success 0 razor ac suc ers spawne 10 <br />Lot Hatch Success Hatch Date Number ofFish Imprinting <br /> (%) Chemical <br />9701 15 970427 363 Phenethyl <br />9702 20 970427 490 Alcohol <br />9703 10 970427 99 <br />9704 1 970427 15 <br />Average 11.5 Total 967 <br /> <br />Problems <br /> <br />Disease proved to be a problem at the facility. Columnaris broke out on May 14, 1997, in two <br />tanks of fish (representing one family lot) that had just been PIT tagged. The timing of the <br />outbreak suggested that infection occurred when the fish were PIT tagged. Once the pathogen <br />was introduced into the hatchery, it proved difficult to isolate and eradicate. The bacteria spread <br />and was ultimately responsible for more than 2,700 mortalities from four family lots. After the <br />pathogen was correctly identified and appropriate treatment was applied, mortalities dropped and <br />we have had no further outbreaks. Several treatments, including Formalin, salt, and Sparaquat 256 <br />baths, were tried before an effective one was found. A combination of Chloramine T and <br />Terramycin baths proved to be most effective. A sanitization procedure has been implemented to <br />lessen the likelihood of outside infection in the future. The procedure includes sanitizing hands <br />and shoes with Sparaquat 256 upon entering the hatchery, and regularly sanitizing the floor and <br />equipment. <br /> <br />Another problem was that the biofilter fails when chemical treatments (e.g., Sparaquat 256 or <br />Chloramine T) or improper back-flushing kill the bacteria that live within the filter.. When the <br />bacteria die, the filter is not able to convert ammonia and nitrite into nontoxic nitrate, creating <br />toxic levels of both ammonia and nitrite. When the filter fails, feeding must be reduced or stopped <br />until the bacteria get reestablished inside the filter. Once they are reestablished, feeding can be <br />resumed gradually. Several actions can be taken to reduce the toxicity of ammonia and nitrite <br />levels while the biofilter recovers. Carbon dioxide can be added to lower the pH of the water <br />which decreases the toxicity of the ammonia. A product called Ammo-lock 2 can be added to the <br />system to instantly detoxify ammonia. Salt can be added to the water, to lower the toxicity of the <br />nitrite. The chloride ions from the salt join with the free nitrite ions which reduces toxicity to fish. <br /> <br />-18- <br />