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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />Table 9. <br /> <br />Lot and source of 1996-year-class razorback sucken stocked into the San <br />Juan River in fall of 1997 <br /> <br />Lot 24 Road Clymers Pond Total <br />9601A 0 230 230 <br />9603A 671 88 759 <br />9610 642 0 642 <br />Total 1313 318 1631 <br /> <br />1997 <br /> <br />On Apri123, 1997, razorback sucker eggs, spawned from broodstock held at Horsethief, were <br />transferred into the hatchery and acclimated. As before, the eggs were bathed in formalin every <br />day to avoid fungal growth. Hatching success varied from approximately 1 % to 20% (Table 10). <br />Of the 17 family lots spawned, only four lots hatched successfully. Poor hatching success may be <br />attributed to a sudden drop in temperature at Horsethief ponds just before spawning. The week <br />before spawning was to take place, water temperature reached 600 F and the: razorback broodstock <br />were ripening. The fish were injected with hormones and they were progressing normally to <br />spawning. However, the day before the fish were to spawn, the weather changed and both the air <br />and water temperature dropped. The air temperature dropped to near freezing and water <br />temperature dropped to 500F. The fish had to be spawned because they were ripe and ready to <br />go. The water temperature at the 24 Road Hatchery was approximately 750 F on the day of <br />spawning and there was no means to cool it down. Although the eggs were tempered before <br />being placed in the hatchery, the 250 difference in water temperature may have been to much for <br />the eggs to adapt to. This large temperature difference between spawning water and the rearing <br />water may have been responsible for the poor hatching success. Another factor in the poor <br />hatching success may have been poor water quality at the hatchery when the eggs were transferred <br />to the facility. The biofilter was not working properly at that time and ammonia and nitrite levels <br />were higher than normal. The high levels of these toxic compounds may have harmed the eggs and <br />led to poor hatching success. <br /> <br />The day before eggs were transferred to the facility, phenethyl alcohol was circulated throughout <br />the facility to imprint the fish for future chemoreception work. The phenethyl alcohol was <br />removed from the system by a combination of evaporation and dilution over a period of 7 to 10 <br />days. <br /> <br />As of December 1, 1997,604 razorback suckers (representing four family lots) are being reared at <br />the 24 Rd. Hatchery. Most will be transferred to Horsethieffor future use as, broodstock. After <br />broodstock requirements are met, excess fish may be stocked into the Gunnison River in 1998. <br /> <br />-17- <br />