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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />(Trichophyra sp.), columnaris (Flexibacter columnaris), black grubs (Neascus sp.) and <br />myxosporean (Myxobolus sp.) take a heavy toll on fish held at Horsethief. The fish often suffer <br />the additional stress of sunburn when they are ill and swimming near the surface. Prophylactic <br />treatments of salt, formalin, or CWoramine T offer some benefits and are done regularly when <br />handling fish, and at first sign of disease. <br /> <br />Nitrogen supersaturation has also been a problem in the past, because the river water is <br />supersaturated with nitrogen before it is pumped into the ponds. This becomes a major problem <br />when the ponds ice over and the nitrogen is trapped in the water. Nitrogen supersaturation has <br />been managed with the use of aerators and reducing the inflow rates to 1 00 gallons per minute or <br />less. The reduced flow rate allows the aerators to release the nitrogen and oxygenate the water. <br /> <br />Predation by herons, coyotes, and other birds and mammals is another problem that arises at <br />Horsethief The predation is often dependant upon disease as predators would not normally have <br />access to healthy fish. When the fish become ill and begin to succumb to a malady, they swim or <br />float to the surface and eventually to the edges of the pond where predators have access to them. <br />This often hides the severity of disease outbreaks as there are no dead or moribund fish to expose <br />the problem. When this happens, no treatment is administered and the problem worsens. Problem <br />animals have been removed from the area. Four of the ponds at Horsethiefhave been covered by <br />nets to reduce avian predation. <br /> <br />24-ROAD HATCHERY <br /> <br />1996 <br /> <br />In May of 1996, razorback brood stock from HorsethiefPonds were spawned and the eggs were <br />transferred into the 24 Road Hatchery and acclimatated. The eggs received a 1,667 ppm formalin <br />bath for fifteen minutes every day until hatch to avoid fungal growth. The razorbacks hatched and <br />began feeding 3 to 5 days after spawning. A total of 13 family lots of fish hatched; 10 for <br />brood stock and stocking and 3 for selenium studies. Hatching success varied from 3% to 95%. <br />Over 29,500 fish were hatched from the 13 lots (Tables 2-3). <br /> <br />Razorback suckers are thought to be able to imprint on synthetic chemicals (Scholtz et al1994), <br />therefore different lots of eggs were subjected to two imprinting chemicals. Some eggs were <br />placed in water treated with 0.056 ppm phenethyl alcohol and some in water with 0.000056 ppm <br />morpholine (Table 3). After hatching the razorbacks were put in tanks without imprinting <br />chemicals and the tanks with chemicals were drained. <br /> <br />After hatching, the fish were fed a razorback diet prepared by Rick Barrows, USFWS, at the <br />Bozeman Fish Technology Center. This razorback diet was produced in an effort to reduce <br /> <br />-13- <br />